肺炎克雷伯杆菌特异性卵黄抗体的制备与活性的-研究.pdfVIP

结论：实验中采用的分离纯化方法可获得高纯度的IgY。所制备的特异性IgY 具有高效价和明显的体外抑菌活性。特异性IgY对小鼠肺炎克雷伯杆菌肺炎有保 护作用。 关键词：特异性卵黄抗体肺炎克雷伯杆菌肺炎 and Evaluationof Yolk PreparationEfficacy Egg KlebsiellaPneumoniae Master candidate：Fan—Li degree kong Zhen Supervisor：ProfessorYu—Hong Major：Pharmacology Abstract chicken yolk Objective：Topreparespecific egg its invitroandinvivo． activity Klebsiellapneumoniae(K．pneumonia)，evaluate was hens、析tll byimmunizing formaldehyde Methods：SpecificIgY produced killedK and water andultrafiltration． dilution，salt by precipitations pneumoniapurified and titerof weredetectedSDS The and by purity IgY Growthinhibitionof atdifferentconcentrationstoK was specificIgY pneumonia Wasestablishednasalinstillation in culturemedium．Pneumoniamodel tested by liquid ofK 1 adoseof100 were dividedinto 011cfu／m1)at 91．Micerandomly pneumonia(2x six and control group(no groupsprotecteddifferently：blank control