《全蝎入药工艺的优化与蝎体蛋白的分离》-毕业论文.docVIP

PAGE w 毕业论文（设计） 全蝎入药工艺的优化与蝎体蛋白的分离 院（系）：海洋学院 专 业：生物工程 学 生： 导 师： 哈 尔 滨 工 业 大 学 w 摘要 沂蒙全蝎是我国的名贵药材，其入药对多种疾病有预防和治疗作用。本实验对传统的全蝎炮制、煎煮入药工艺进行改进，并对活蝎蝎体蛋白进行了分离纯化。 全蝎的炮制作为传统的制药技术在很大程度上决定着全蝎药材的质量。本实验采用薄层色谱法对全蝎炮制技术进行了优化，得出将全蝎放入清水或2%淡盐水中浸泡4 h，然后放入约10-20倍量沸开水中煮沸5 min的最优工艺。 全蝎煎煮工艺的优化是以蝎毒蛋白的含量为指标，采用考马斯亮蓝G-250染色法测定其含量，用正交设计法进行提取工艺条件的筛选，最终得出了将全蝎粉末用14倍量水浸润15分钟，隔水煮沸60 min的最佳工艺条件。 采用水提法获得活蝎蝎体的总蛋白，通过SephadexG-75凝胶色谱和SDS，得到活蝎蝎体各蛋白组分。与传统的入药蝎体及蝎尾毒蛋白做比较，可知活蝎蝎体比入药蝎体多出分子量为46000和8000的两个蛋白组分，并具有蝎尾毒所缺乏的大分子蛋白。这一结果可以为蛋白质药物研究提供一组基础数据，并对全蝎综合利用方案的提出具有理论指导作用。 关键词：沂蒙全蝎，正交设计，蝎体蛋白，分离纯化，凝胶层析 Abstract Yimeng Scorpion is a kind of rare medicinal herbs. Its usage for medical purpose can produce preventive and restraining effect to some diseases which are dangerous to human beings. This experiment starts with traditional and innovative technics. When trying toimprove the traditional ways of Scorpion processing，and decoct into medicine, it also tries to separate and purify the protein of living scorpions. As a traditional pharmacy technics, processing decides the quality of traditional Chinese medicinal materials and influences the HYPERLINK /clinical/ \t _blank clinical trials。This experiment uses TLC to detect the quality of processing scorpion roughly and get feasible processing technics: put scorpion into water and immerse about 4 hours,then boil about 5 min in the boiled water. The optimization of decocting scorpions is targeted by the content of scorpion venom protein. Using Coomassie brilliant blueG-250 staining to measure its content and study the withdrawing technique of decocting scorpion. Then using orthogonal design to select the withdrawing conditions and get the best technique condition:put the power of scorpions into water and immerse 15 min,then boiled about 60 min. The separation and purification of scorpions is that take the living scorpions, break then up and get total protein by waterextract method. Then, through SephadexG-75 gel chromatography and SDS, getting component of sc