系统性红斑狼疮患者外周血单个核细胞超保守区域转录表达水平剖析.docVIP

系统性红斑狼疮患者外周血单个核细胞超保守区域转录表达水平剖析 　　【摘 要】目的：探讨超保守区域转录因子与系统性红斑狼疮的相关性。 　　【关键词】 红斑狼疮，系统性；单个核细胞；T-UCRs；生物标志物 　　doi：10.3969/j.issn.2095-4174.2014.04.006 　　Analyzing the Expression Level of the Ultra Conservative Region of the Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus 　　QIU Jin-jun，SUI Wei-guo，CAO Cui-hui，HE Hui-yan，ZHANG Yang，DAI Yong 　　【ABSTRACT】 Objective：Systemic lupus erythematosus is a systemic autoimmune disease regulated by a variety of genes.T-UCR is an important part of the long non coding RNA transcribed from the 481 extreme conservative regions in the human genome，which will change the expression pattern in many kinds of cancers such as leukemia，liver cancer，colon cancer，neuroblastoma and so on.Method：T-UCR chip technology was used to analyze the expression level of the ultra conservative region of the peripheral blood mononuclear cells in 15 patients with systemic lupus erythematosus and 15 healthy people.Results：Compared the group with systemic lupus erythematosus with the healthy control group，the expression level of 18 T-UCRs（uc.285+，uc.234+，uc. 　　102+，uc.341+，uc.267+，uc.221-，uc.290- and uc.90+） up-regulated and the expression level of 29 T-UCRs down-regulated，among which the expression of uc.186- was the most significant in the corresponding ultra conserved region （p = 3.29，FC=-38.648 61）and HNRNPH1 was its related gene.Conclusion：GO analysis showed that uc.186- might be a potential biomarker for diagnosis and prognosis of systemic lupus erythematosus，and HNRNPH1 might be the potential key gene of lupus erythematosus system. 　　【Keywords】 lupus erythematosus，systemic；mononuclear cells；T-UCRs；biological marker 　　系统性红斑狼疮（systemic lupus erythematosus， 　　SLE）是一种与弥漫性结缔组织相关的典型的全身性自身免疫病[1]，其特征在于发生免疫炎症。SLE的发病机制复杂[2]，涉及家族遗传、免疫和环境因素等，可能会导致组织和器官的损害，其中最复杂的是肾功能损害[3]。在人、小鼠和大鼠基因中极度保守的长段非编码基因区域，命名为超保守区域（ul-traconserved regions，UCRs）。UCR为长约200～779 bp的序列，最初于2004年由Bejerano G发现[4-5]。UCR常位于肿瘤相关基因区域或脆性位点。UCR编码的一群特殊的非编码RNA（ncRNA），称为