大豆异黄酮对大鼠脑缺血再灌后神经细胞凋亡的影响及机理探讨-病理学与病理生理学专业论文.docx

组 Ca2+-Mg2+-ATP 酶及 Na+-K+-ATP 酶的活性明显低于 Sham 组；SI 组 Ca2+-Mg2+-ATP 酶及 Na+-K+-ATP 酶的活性显著高于 IR 组，各组间差异显著 （P0.05）。5、半定量 RT-PCR 检测结果显示：Sham 组脑组织 bcl-2 mRNA， bax mRNA 表达较低；IR 组 bcl-2mRNA 表达略增高、bax mRNA 表达显著高于 Sham 组，而 bcl-2/bax 比值显著低于 Sham 组；SI 组 bcl-2 mRNA 表达高于 IR 组， bax mRNA 表达较 IR 组相比明显降低，而 bcl-2/bax 比值显著高于 IR 组。各组 间差异显著（P0.05）。6、免疫组化检测结果显示：Sham 组仅见少量的胞浆淡 染呈棕黄色 Caspase-3 阳性细胞表达；而 IR 组，Caspase-3 阳性细胞表达呈强阳 性且阳性细胞数目明显增加；SI 组 Caspase-3 阳性细胞的染色及数目明显低于 IR 组。各组间差异显著（P0.05）。 结论：1、大鼠全脑缺血再灌注损伤时 Ca2+-Mg2+-ATP 酶及 Na+-K+-ATP 酶的活 性降低；促凋亡基因 bax mRNA 表达升高而抑凋亡基因 bcl-2 mRNA 降低； Caspase-3 蛋白表达增高。 2、大豆异黄酮可减轻大鼠全脑缺血再灌注损伤而发挥神经保护作用 3、大豆异黄酮神经保护的机理可能有： 1）提高 Ca2+-Mg2+-ATP 酶及 Na+-K+-ATP 酶的活性,调节细胞内外的离子平衡， 是减轻细胞内钙超载的因素之一。 2）通过上调抑凋亡基因 bcl-2，下调促凋亡基因 bax，及增加 bcl-2/bax 比值。 3）间接调控 Caspase-3 的表达而抑制神经细胞凋亡。 关键词：脑缺血再灌注损伤；凋亡；大豆异黄酮；bcl-2；bax；钠钾 ATP 酶；钙 镁 ATP 酶。 To explore the effects of soybean isoflavone on rats，nerons after cerebral ischemia/reperfusion and relative mechanisms Abstract Objective: 1. To observe the effects of soybean isoflavone (SI) on the apoptosis of nerons in rats after global cerebral ischemia/ reperfusion (IR) injury. 2. To explore the mechanisms of neural protecting effects of SI on cerebral isehemia/reperfusion injury. Methods: Sixty adult Sprague-Dawley rats were randomly divided into three groups: sham operation group, IR group, SI pretreating group,each group has twenty ones. The SI pretreating group was given intragastric administration of SI (120 mg/kg) for three weeks, while the other groups were inseaded by normal saline of equal volume, then they were all operated on the 22nd day, estabalished global cerebral ischemia- reperfusion injury model by blocking three vessels, the sham operation group was only operated, non-ischemic. The other groups were one hour later, then irrigated them for 1 h, monitoring the changes of EEG, observed the pathological changes of the cortex and hippocampus of the brain tissues under the light microscrope and the ultramicrostructure of the nerons