耳针激活胆碱能抗炎通路抑制糖尿病血管并发症炎症反应机制研究-药理学专业论文.docxVIP

三峡大学硕士学位论文 三 峡 大 学 硕 士 学 位 论 文 PAGE VI PAGE VI (5)耳针刺激前离断针刺侧颈部迷走神经或尾静脉注射六烃季铵，α-银环蛇毒素均 可抵消耳针保护海马区微血管的效应，但耳针刺激前尾静脉注射阿托品对耳针效应无 明显影响。 结论： (1)促炎因子的异常升高和抗炎因子的明显降低伴随2型糖尿病的发病过程，糖尿 病可能是一种慢性炎症性疾病； (2)耳针刺激迷走神经分布区穴位CO11可抑制2型糖尿病炎症反应，减低糖尿病大 鼠胰岛素抵抗指数，产生保护2型糖尿病大鼠脑微血管的效果； (3)耳针效应的发挥依赖完整的迷走神经，可能是通过迷走神经耳甲分支介导， 激活胆碱能抗炎通路实现的； 关键词：耳针 胆碱能抗炎通路 迷走神经 糖尿病 胰岛素抵抗 Abstract Objective To observe the efficacy of electro-acupuncture at auricular concha acupoints (EAACA) on modulate the inflammatory parameters in the blood of induced diabetic rats, aimed to explore the cholinergic anti-inflammatory mechanism about the prevention or cure of diabetic vascular complication through auricular acupoint stimulating, reveal the scientific connotation of auricular acupuncture, and to provide more strategic choices for clinical prevention of diabetic vascular complication. Methods. (1)10 male Wistar rats were equally and randomly picked from 80 rats as group 1 (normal group), which were given normal diet; the rest of 70 rats were given high fat and sugar and streptozotocin injection, aim to produe type 2 diabetic rat model; (2)43 rats that were successfully induced type 2 diabetic were equally and randomly divided into six groups: group2 (model group): without any treatment only for the model control; group3 (treatment group): received electro-stimulation at auricular concha acupoints(CO11) for 30 min at 1 mA, 2 Hz, continually lasted for 10 days; group 4 (vagotomy group): received vagotomy ipsilaterally before auricular acupuncture; group 5(atropine group), group 6(Hexamethonium group), group 7(α-bungarotoxin group): injecting atropine, Hexamethonium, α-bungarotoxin via tail vein before acupuncture respectively; (3)All animals were fasted 12h before the last ear acupuncture. Rats were anesthetized immediately at the end of the last needle ,then collected blood by Carotid, obtained serum by centrifugation, measured inflammatory cytokine TNF-α、IL-6、CRP and IL-10 levels by sandwich ELISA method; (4)Fasting i