大壁虎中脑脊柱侧弯运动亚区的研究-机械设计及理论专业论文.docx

摘 要 为深入探索大壁虎（Gekko gecko）中脑运动调控功能，通过对头部固定的大壁虎（n=10） 中脑脑区采用不锈钢微电极施加电刺激，发现对中脑不同深度脑区的电刺激能诱导出脊柱侧弯、 四肢动等运动反应。在实验过程中，一些刺激针道在中脑围脑室灰质（或在中央灰质）诱导出 脊柱同侧弯曲，而在中脑腹侧被盖诱导出脊柱相反方向侧弯反应。通过对刺激脑区的组织学检 验，本研究确定了大壁虎中脑内控制脊柱同侧弯以及对侧弯运动相关核团的大致分布区域，初 步比较了两种核团在细胞构筑上的差异。 为进一步验证运动相关脑区的功能，与电刺激实验研究结果相比照，本文进行了少量例数 的大壁虎中脑急性谷氨酸化学刺激实验，重点研究了急性电刺激实验中脊柱同侧弯和脊柱对侧 弯相关脑区。结果发现，电刺激实验中脊柱对侧弯曲位点在谷氨酸刺激下可靠地诱发了相同的 动作模式，证实了脊柱对侧弯运动核团为谷氨酸能核团；脊柱同侧弯曲动作在谷氨酸刺激条件 下没有被诱发，表明神经递质谷氨酸钠对脊柱同侧弯运动相关核团内的神经元不具有兴奋性。 在大壁虎急性刺激研究之后，参照大壁虎颅骨外形特征，本文进行了适用于大壁虎的微电 极推进装置的初步设计，得出两套装置方案，为后续研究清醒状态下电刺激大壁虎中脑不同运 动亚区对动作行为的影响奠定了基础。 关键词：大壁虎，中脑，脊柱同侧弯曲，脊柱对侧弯曲，电刺激，化学刺激 ABSTRACT To study thoroughly the locomotion modulation function of Gekko gecko’s midbrain, 10 head fixed Gekko geckos were used in electrical mesencephalon stimulation experiments. During experiments the animal’s mesencephalic areas were electrically stimulated via micro stainless steel electrodes and its responses during stimulation were observed. The results implied that locomotion responses such as lateral spinal bending, limbs’ movements could be elicited in different parts of gecko’s mesencephalon.Some stimulation tracts elicited ipsilateral spinal bending in periaqueductal gray area(or in central gray area) while elicited contralateral spinal bending at ventral tegmentum. The approximate distribution areas of two types of nucleus that modulate inversus spinal bending movements are definited by histological analysis to stimulated mesencephalic region.This thesis preliminarily compared the diferences of above two types of nucleus in cytoarchitecture. To further test and verify the function of midbrain’s subregions relative to movement, comparing with the result of electrical mesencephalon stimulation study of Gekko gecko,two cases of sodium glutamate stimulation experiments were carried on.Areas relative to ipsilateral spinal bending and contralateral spinal bending during electrical mesencephalon stimulation were studied.The result implied that contralateral spinal bending sites got from e