分子印迹电化学发光传感器的研制及应用-分析化学专业论文.docx

II II PAGE PAGE III 的方法制备得到分子印迹电化学发光膜修饰电极。邻苯三酚与茜素红在电极上发生一系 列化学反应，产生电化学发光信号，而强力霉素能够增强邻苯三酚-茜素红体系的电化 学发光强度，从而达到间接测定兽药残留强力霉素的目的。随着重新吸附样品中的 DC 浓度不断增加，体系的 ECL 强度不断增强。方法的检测范围为 2×10-10 mol/L ~ 500×10-10 mol/L，检出限为 5.17×10-11 mol/L，该传感器应用于市售的鲤鱼样品中的 DC 残留检测， 结果令人满意。 （5）针对手性物质分析测定的难点，引入 6-SH-β-CD 提高分子印迹传感器的识别能 力，研制了新型 MIP-ECL 传感器用于左旋咪唑（LMS）的检测分析。首先在金电极表 面自主装得到 6-SH-β-CD 修饰电极，然后在该修饰电极上以邻苯二胺为功能单体，LMS 为模板分子，制备得到分子印迹膜。检测过程中，LMS 与葡萄糖氧化酶（GOD）标记 的 LMS（GOD-LMS）发生竞争反应。经过竞争反应后，印迹膜上的 GOD-LMS 不断被 样品中的 LMS 取代，底液中鲁米诺-葡萄糖发光体系的 ECL 强度不断减小，以此建立了 一种检测 LMS 的新方法。方法的检测范围为 8×10-11 mol/L ~ 4000×10-11 mol/L，检测出 限为 2.12×10-11 mol/L。由于 6-SH-β-CD 能够极大的提高分析印迹膜的识别能力，相对 于传统分子印迹传感器，该传感器表现出了有着更好的识别能力。 关键词：分子印迹技术；酶放大；罗丹明 B 放大；量子点表面分子印迹；分子印迹发光 膜；手性识别 Abstract Abstract Molecular imprinting technique (MIT), as a molecular recognizing technology, has the features such as preordainment, specific recognition and wide application range. The sensor based on the MIT has the advantages such as high sensitivity, low determination limit and good selectivity etc. In the thesis, the MIT and the electrochemiluminescence (ECL) detection with high sensitivity and controllability were used together to fabricate a series of novel MIP-ECL sensors for the determination of agricultural chemicals and veterinary medicine residual. Enzymatic amplifying effects, organic dyestuff derived amplifying effect and molecular imprinting on surface of quantum dots were also used in the assay. The main results are listed as follow: A novel MIP-ECL sensor for the determination of isoproturon (IPU) was fabricated. In the assay, IPU was competed with glucose oxidase labeled IPU (GOD- IPU). Then the GOD- IPU remained on the molecular imprinted membrane could catalyze the glucose in the solution and form H2O2, which then reacted with the luminol in the solution and produce ECL signals. The intensity of the ECL signals decreased with the increase of the IPU concentrations. The detection range was 9×10-11 mol/L ~ 510×10-11 mol/L, with a detectio