番茄可溶性固形物及相关性状的动态QTL定位-生物化学与分子生物学专业论文.docxVIP

Abstract Object：Tomato SSC solid content is a complex and important quantitative traits, is a nuclear inheritance, is not affected by the cytoplasm, showing additive effects. SSR markers are used for constructing the genetic linkage map of tomato, and tomato SSC solid content and associated quality traits are for initial dynamic QTL. This provides a theoretical basis of tomato varieties for our marker-assisted selection breeding and quality trait loci for positional cloning. Methods: This study used 166 F2 populations from the cross between TD22 and HT-1-1-1-1 which are processing tomato and fresh eating tomato respectively, derivatived from single seed for dynamic QTL research, SSR molecular markers are used for studying QTL. The expression of SSC is a dynamic process, in order to fully understand the QTL genetic effects, we should not only know their genetic given point in time the role, but also to understand their dynamic expression. Analysis using dynamic models to analyze the phenotypic values of SSC solids with time-varying function model, to detect changes in dynamic traits influence the process of major genes. In this study, three tomato fruit development period (green ripe stage, yellow ripe stage and the red ripe stage) for dynamic positioning QTL. Results: 1.Though the analysis of quality traits of F2:3 family lines which are derived from TD22 and HT-1-1-1-1, every quality trait which is controlled by multiple genes is in accordance with in line with normal quality traits. There are some correlation among the traits of tomato quality. SSC are positively correlated with SSC sugar but negatively correlated with fruit weight (FW) in green ripe stage. SSC are positively correlated with SSC sugar and organic acid in yellow ripe stage. SSC are positively correlated with SSC Sugar and organic acid but negatively correlated with fruit weight in red ripe stage. Through the progressively lines regression analysis, we constructed lines regression equation. Green r