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PAGE PAGE 2 阻断内源性P75NTR对阿尔茨海默病小鼠海马神经干细胞增殖与学习记忆的影响 高长越，方传勤，许志强，李 静，邓 娟，王延江，周华东 （400042 重庆，第三军医大学大坪医院野战外科研究所神经内科） = \* MERGEFORMAT [摘要] 目的 研究阿尔茨海默病(Alzheimer’s disease, AD)小鼠侧脑室注射p75NTR胞外段(extracellular domain of p75NTR, p75NTR-ECD) 阻断内源性p75NTR激活后对海马神经干细胞增殖和学习记忆影响。 = \* MERGEFORMAT 方法 选取12月龄APP/PS1双转基因AD小鼠16只，注射组（n=8）连续7 d 侧脑室注射p75NTR-ECD（1 μg/d），对照组（n=8）向侧脑室注射等量生理盐水，Morris 水迷宫检测空间记忆，腹腔注射BrdU检测海马齿回神经干细胞增值变化。 = \* MERGEFORMAT 结果 与对照组比较， = \* MERGEFORMAT 注射组小鼠海马齿回BrdU阳性细胞数显著增[（142.01±18.12）加（vs （286.39±26.17），P 0.01]，行为学检测显示逃逸潜伏期明显缩短[（39.58±6.19） s 加（vs （30.39±4.31） s, P 0.05]、穿过原平台位置次数和在原平台象限探索时间百分率显著增加请补充行为学检测显示逃逸潜伏期明显缩短2组具体数据（含单位）及P值[（2.54±0.32）vs（3.72±0.45）；（30.42±4.31）% vs （39.58±6.19）%；P 0.05]。 = \* MERGEFORMAT 结论 阻断内源性p75NTR激活可促进AD小鼠海马神经发生并改善AD小鼠学习记忆。 请补充行为学检测显示逃逸潜伏期明显缩短2组具体数据（含单位）及P值 = \* MERGEFORMAT [关键词] = \* MERGEFORMAT 受体, 神经生长因子类p75NTR-ECD；阿尔茨海默病；海马；学习；记忆；细胞增殖；小鼠神经发生 [中图法分类号] [文献标志码] A = \* MERGEFORMAT Effect of p75NTR-ECD on learning and memory of Alzheimer’s disease mice and NSCs proliferation changes in hippocampus = \* MERGEFORMAT Gao Changyue,Fang Chuanqin, Xu Zhiqiang,Li Jing, Deng Juan, Wang Yanjiang,Zhou Huadong = \* MERGEFORMAT （Department of Neurology ,Daping Hospital,Third Military Medical University,Chongqing 400042,China） = \* MERGEFORMAT [Abstract]　Objective 　 = \* MERGEFORMAT To observe the effect of p75NTR-ECD on learning and memory of APP/PS1 transgenic Alzheimer’s disease (AD) mice and neural stem cells(NSCs) proliferation changes in the hippocampus. = \* MERGEFORMAT Methods In the experimental group ,p75NTR-ECD was injected into lateral cerebral ventricle of 12-months-old AD mice for 7 days（1μg/d）,and saline was injected in the control group. BrdU immunohistochemistry was used to oberve NSCs proliferation in hippocampus and Morris Water Maze test was performed to detect the praxiology of AD mice. = \* MERGEFORMAT Results 英文同中文修改 NSCs proliferation in the hippocampus was obv