替换HIV21衣壳蛋白基因SHIV的构建及其活性测定.PDFVIP

替换 HIV1 衣壳蛋白基因 SHIV 的构建及其活性测定 1. 2 2 2 2 2 1 ,3 朱义鑫 ,刘 　畅 ,乔文涛 ,陈启民 ,耿运琪 , 曾　毅 ( 1. 中国疾病预防控制中心病毒病预防控制所 ,北京 100052 ; 2 . 南开大学生命科学院 ,天津 30007 1 ; 3 . 北京工业大学 ,北京 100022) Construction and Characterization of SHIV Carrying HIV1 Capsid ZHU Yixin1. 2 ,L IU Chang2 ,Q IA O Went ao2 ,C H EN Qimin2 , GEN G Yunqi2 ,ZEN G Yi1 ( 1. N at ional I ns t it ute f or Vi ral D isease Cont rol an d P revent ion , CD C, B eij i ng 100052 Chi an; 2 . Col leg e of L if e S ciences , N an ) k ai Uni vers ity , Ti anj i n 30007 1 Chi na; 3. B ei i ng Uni vers ity of Technol ogy , B eij i ng 100022 , Chi na Abstract : SH IV ( SIV/ H IV chimeric vir u s) p roviral DN A car rying Human immuno deficiency vir u s t yp e 1 ( H IV1 H XBc2) cap sid wa s con st r uct ed on t he backbone of S i m i an i m m uno def icien cy v i r us ( SIV mm239) . The gene exp re ssio n of chimeric vir u s coul d be det ect ed in 293 T cell s t ran sfect ed by SH IV p roviral DN A . Chimeric SH IV virion s were al so obt ained in t he sup er nat ant of t ran sf ect ed 293 T cell s. The se virion s have co mp let e st r uct ure s ,inclu ding geno mic RN A , re ver se t ran scrip t a se , core p rot ein s an d envelop e wit h glycop rot ein s. The chimeric Gag p recur sor coul d be app rop riat ely cleaved and lead to t he co nfor mation of spin dle core in t he mat ure vir u s p article s. They coul d ab sor b and ent er into M T4 cell s an d co mp let e t he cour se of rever se t ran scrip t