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植物生长调节剂对香樟叶片生理代谢影响
植物生长调节剂对香樟叶片生理代谢影响
摘要:试验探讨了低温处理后,植物生长调节剂对香樟叶片细胞膜透性和保护酶活性等生理代谢的影响,以期客观地评价香樟的抗寒力和引种到暖温带地区应用的可行性,为北方地区引种栽培提供理论依据。结果表明,低温处理后,香樟叶片的细胞膜透性和保护酶活性均有不同程度的变化,当植物生长调节剂喷施的质量浓度达到300 mg/L时,香樟叶片的SOD、POD、CAT活性明显提高,相对电导率、Pro和MDA含量相对下降。
关键词:香樟;植物生长调节剂;细胞膜透性;保护酶活性
中图分类号:S792.23;S482.8;S718.3 文献标识码:A 文章编号:0439-8114(2012)02-0329-03
Effects of Plant Growth Regulators on Physiology and Metablism of
Cinnamomum camphora Leaves
WU Juan1,CHEN Jin-miao2,MA Chang-jiang1
(1. School of Life Science, Anqing Teachers College,Anqing 246011,Anhui, China;
2. Anqing Municipal Engineering Management Department, Anqing 246001, Anhui, China)
Abstract: The effects of plant growth regulators on the leaf membrane permeability and protective enzyme activity in cold treated Cinnamomum camphora(L.) Presl were discussed to provide a theoretical basis for the feasibility of introduction of this plant into northern area. The results showed that the physiological indicators including SOD, POD, CAT, electrolyte leakage rate, Pro, MDA were influenced in some degree when different concentration of plant growth regulators was sprayed on the leaves. SOD, POD and CAT activity was the highest and the relative conductivity, Pro and MDA content was the lowest when the concentration of plant growth regulator was 300 mg/L.
Key words: Cinnamomum camphora(L.)Presl ; plant growth regulators; membrane permeability; protective enzyme activity
香樟[Cinnamomum camphora(L.)Presl]为樟科(Lauraceae)樟属(Cinnamomum Trew)常绿乔木,性喜温暖湿润的气候环境,不耐寒冷,在我国中东部地区分布较多[1]。该树种枝叶茂密、冠大荫浓、树姿雄伟,能吸烟滞尘、涵养水源、固土防沙和美化环境,是城市绿化的优良树种[2]。然而在北方地区,由于引种栽培过程中各种逆境条件对引进树种如香樟会产生许多不利的影响,因此,探讨引进树种增强抗逆性的技术管理措施,对丰富引种地树种资源有着重要的意义。
植物生长调节剂对植物的抗寒性有明显的影响,但以往的研究多集中在粮食作物上[3-5],对城市行道树如香樟的叶片细胞膜透性和保护酶活性的影响却鲜见报道。因此,试验以香樟幼树为材料,探讨了植物生长调节剂对香樟幼树抗性生理的影响,即对叶片细胞膜透性和保护酶活性的影响,旨在为香樟在北方寒冷地区引种栽培和安全越冬提供理论依据。
1 材料与方法
1.1 材料的选择处理
试验地位于安徽省安庆市菱湖公园。试验材料选取生长环境相同、长势相同且树体大小相当的一年生香樟幼树,于2009年11月1日进行-10℃的低温处理12 h后移栽。试验使用的植物生长调节剂为多效唑和6-苄氨基嘌呤混合液,按质量比1∶1配比,设6个处理水平
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