抵抗素对小鼠静脉血栓形成的影响及血管内皮功能的调节-内科学(心血管内科)专业论文.docx

汕头 汕头大学医学院硕士学位论文 汕头 汕头大学医学院硕士学位论文 III III IV IV Abstract Background and objective: In recent years, the incidence of metabolic syndrome which is mainly featured as central obesity and insulin resistance was increased year by year. Patients with metabolic syndrome were prone to in a pro-thrombotic state, which may increase the risk of arterial and venous thrombosis diseases. But the pathogenesis of thrombus was not elucidated fully. Resistin is an important cytokine served as intermediate link between obesity and insulin resistance, which has a proinflammatory role. Our previous clinical observation and vitro experiment found that resistin was positive correlation with the factors of thrombosis, which maybe was one of the mechanisms that leading to the metabolic syndrome with the complication of thrombotic diseases easily. Based on it, this study which was used the high-fat diet induced obese mice model is performed to discuss the effect of resistin both from endogenous and exogenous on murine venous thrombosis and vascular endothelial function. Methods: This study was conducted on 5-6 weeks old C57BL/6 male mice (about 40-50). Mice were divided into two groups randomly: normal rodent diet group (n=24) and high-fat diet group (n=24), which were fed with normal rodent diet contained 10% calorie from fat and high-fat diet contained 60% of calorie from fat, respectively. After mice were fed with normal rodent diet or high-fat diet for 10-12 weeks, the high-fat diet induced obese mice were produced. When mice were about 16-18 weeks old, the normal rodent diet group were randomized into saline group (ND+NS; n=12), resistin group (ND+RE; n=12); the High-fat diet group were randomized into saline group (HD+NS; n=12) and resistin group (HD+RE; n=12). Mice in the saline group received 0.9% saline through intraperitoneal injection for 2 weeks; the resistin group received an infusion of recombinant resistin (33.33μg/kg/d) through intraperitoneal injection for 2 weeks. And then