复脑灵对血管性痴呆大鼠学习记忆功能及海马细胞凋亡的影响中西医结合;临床专业论文.docx

PAGE PAGE 2 较模型组明显减少；④模型组凋亡指数显著高于假手术组(P﹤0.01)。复 脑灵口服液组凋亡指数显著低于模型组(P﹤0.01)，而且复脑灵口服液相 邻组间两两比较无显著性差异 (P0.05)，不相邻两组比较具有统计学意 义(P0.01)。 结 论：复脑灵口服液对缺血再灌注损伤大鼠的学习和记忆能力方面具 有提高和改善作用，对缺血再灌注损伤大鼠脑组织内海马细胞凋亡具有 明显的抑制作用。 关键词: 复脑灵口服液；神经行为学；细胞凋亡 The effection of Funaoling Oral Liquid on the learning and memory function of vascular dementia rats and hippocampal apoptosis Abstract Objective: to further explore the role of Funaoling Oral Liquid in the protection of nerve cells and its mechanism by observing its effection on the learning and memory function of Vascular Dementia model rats and hippocampal apoptosis. Methods: produce Vascular Dementia model rats by the method of repeatedly carotid occlusion with non-invasive arterial folder combined with intraperitoneal injection of sodium nitroprusside to reduce blood pressure in rats.To 50 healthy male and female SD rats were equally divided between into 5 groups randomly, with each group 10 rats: sham-operated group (A group), model group (B group) of Funaoling Oral Liquid low-dose group (C group), middle dose group (D group), high dose group (E group). Three days after surgery each group of rats will be given oral administration once each day, for 21 Continuous days. The day after the cessation of administered, conducted each group water maze experiments with MWM test and did positioning navigation test (place navigation) for five consecutive day. The sixth day began to do space exploration test (spacial probe test). After the experiment, decapitated, taken the brain, studied about the brain tissue with HE staining, situ labeled apoptotic cells with TUNEL technique. Results: Water maze place navigation experiments showed that the escape latency of model group changes gently in the 5 days, the latencies of drug treatment groups become significantly shorter with the increase in the number of training days. Escape latency of model group shows a significant difference PAGE PAGE 4 with other groups (P 0.05). The mean escape latencies