低强度循环载荷对兔髌骨髌腱结合部骨微细结构的影响-体育学专业论文.docx

低强度循环载荷对兔髌骨髌腱结合部骨微细结构的影响 低强度循环载荷对兔髌骨髌腱结合部骨微细结构的影响 万方数据 万方数据 ABSTRACT Purpose: The injury of bone-tendon junction has a close relationship with the local stress. Bone structure from the bone-tendon junction will affect formation and healing precess of the injury. At the base of establishing a model of making bone-tendon junction to be injuried with cyclic loading, this study compares the impacts of two different ways to bone-tendon junction injury, rest and low intensity cyclic loading. Micro-CT is used to assess the bone structure from bone-tendon junction of the two methods. The result will show which recovery method is more rational for bone-tendon junction after chronic injury. Method: 19 matual famele New Zealand rabbits (18weeks, weight 2400±200g) were randomly divided into control croup(n=10),rest recovery group(RR, n=7),low intensity cyclic loading group(LL,n=7). Animals from two experimental groups receiveing unified cyclic loading stimulation with 30% max muscle contraction in first 4 weeks. The loading intensity is 7.06 士 0.71（N）, 2 hours per time, 3 times a week, total 4 weeks. After that, Rest recovery group stopped and change cyclic load to 4.2N/S for low intensity cyclic loading group, experimental time is similar with before. Tissues were drawed at 8 weeks. Firstly, proceeding Micro-CT scan reconstruction and followed by HE stained tissue section. The result of Micro-CT is used to evaluate the micro-structure of bone trabecula and observing hyphology change of bone-tendon junction with HE section. Result: Histological observation shows that compared with CON group, there are disordered arrangement of tendon cells, cells aggregation, hyaline and tide line becomes unclear or disappeared in LL group and RR group. In 论文随机验证编号：BT51938203870 VI the zone uncluded bone, the value of TV、BV、BS、BS/BV、BS/TV from CON group、LL group and RR group has no significant difference. BV/TV has significant difference between RR group and CON group. In bone zone, the value o