独活提取物抑制柑桔病原真菌活性成分的分离与鉴定。投稿20111116.doc.docVIP

独活提取物抑制柑桔病原真菌活性成分 的分离与鉴定 李敏敏1.2，胡军华1,项目资助：国家科技支撑计划（2006BAD22B03-B4）；重庆市自然科学基金（CSTC,2008BB1272）作者介绍：李敏敏，女，硕士研究生，植物源杀菌剂方向通讯作者 ：胡军华，电话：（023 E-mail: dhujh@ ， 项目资助：国家科技支撑计划（2006BAD22B03-B4）；重庆市自然科学基金（CSTC,2008BB1272） 作者介绍：李敏敏，女，硕士研究生，植物源杀菌剂方向 通讯作者 ：胡军华，电话：（023 E-mail: dhujh@ （1 西南大学柑桔研究所，2 西南大学园艺园林学院；重庆,400712） 摘要：采用生长速率法、溶剂萃取、硅胶柱层析、气相色谱-质谱联用（GC-MS）等方法研究独活乙醇提取物对3种柑桔贮藏病害病原真菌的抑菌作用，并分离和鉴定其抑菌活性成分。结果表明，独活乙醇提取物对3种柑桔病原真菌均有很好的抑制效果，对柑桔炭疽菌的抑制效果最佳。GC-MS鉴定B2组分中含有4种香豆素类化合物—异佛手柑内酯、牛防风素、茴芹内酯、异茴芹内酯。异佛手柑内酯、茴芹内酯、异茴芹内酯三种化合物对柑桔炭疽菌的EC50均在40-50ug/ml之间，其中茴芹内酯的抑制活性最好，其EC50为41.5216 ug/ml。光学显微镜下观察发现，茴芹内酯处理会导致病原菌菌丝体分支增多，菌丝畸形等变化。 关键词：独活；抑菌活性；分离鉴定 Isolation and identification active ingredients of Radix Angelicae Biseratae extracts against citrus fungus pathogens LI Min-min1,2 , HU Jun-hua1, *, HE-Lei1,2, YAO Tin-shan1, RAN Chun1 , LI Hong-jun1 LIU Hao-qiang1 (1.Citrus Research Institute, Southwest University. 2.College of horticulture and landscape architecture, Southwest University; Chongqing ,400712, Abstract:The antifungal activity of ethanol extract and active ingredients of Radix Angelicae Biseratae were studied by using mycelial growth, Solvent extraction, column chromatography and GC-MS analysis. The results showed that the ethanol extract presents the highest inhibitory activity against Colletorichum gloeosporioides. B2 active ingredients were analyzed by GC-MS and the results showed that bioactive compounds of ethanol extract of Radix Angelicae Biseratae has 4 compounds i.e isobergapten, sphondin, pimpinellin and isopimpinellin. Antifungal virulence of isobergapten, pimpinellin and isopimpinellin was 40-50ug/ml and the EC50 of pimpinellin was 41.5216 ug / ml, best in three compounds. Pimpinellin could induce C.gloeosporioides to become more mycelium branches, deformity and so on. Keywords: Radix Angelicae Biseratae ; antifungal activity; Isolation and identification 柑桔贮藏期间易发生多种病害，最重要的是青霉病和绿霉病，其次是炭疽病、黑腐病、褐色蒂腐病等，常引起果实腐烂，严重影响贮藏及外销，造成重大的经济损失[1]。生物农药具有无公害、低残留、高安全性、高生物活性及