禽大肠杆菌毒力岛主要摄铁基因及生物膜的检测.PDFVIP

安徽农业大学学报, 2016, 43( 1): 47-50 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x018 网络出版时间：2015-12-24 15:00:37 [URL] /kcms/detail/34.1162.S1500.036.html 禽大肠杆菌毒力岛主要摄铁基因及生物膜的检测 * 汪 凯，王 琦，潘 玲 ，刘红梅，周 杰 (安徽农业大学动物科技学院，合肥 230036) 摘 要：以临床病禽分离17 株E. coli 为研究对象，分别检测分离菌株的毒力岛中irp2、irp3、irp4、irp5 和生 物膜形成能力。先用irp2 PCR 扩增检出7 株致病性大肠杆菌，并对其进行ERIC-PCR 分型 ，结果分为2 个类型。 又对分离株进行irp3、irp4 和irp5 基因检测 ，结果分离株中irp2、irp3、irp4 和irp5 的阳性检出率分别为41.2% 、 64.7%、29.4% 和 35.3% ；最后对分离株进行生物膜形成能力的检测，结果携带irp2 的阳性菌株都有较强的生物膜 形成能力。结果提示，禽致病性大肠杆菌的HPI 中irp2 与生物膜的形成有一定的关联。 关键词：致病性大肠杆菌 ；irp2 ；ERIC-PCR ；生物膜 中图分类号：S852.6 文献标识码：A 文章编号：1672352X (2016)0 1004704 Detection of main iron-uptake genes of high pathogenicity island and bacterial biofilm among E. coli WANG Kai, WANG Qi, PAN Ling, LIU Hongmei, ZHOU Jie (School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036) Abstract : Seventeen E. coli strains of clinical avian E. coli were used to detect irp2, irp3, irp4 and irp5 located in HPI and their biofilm formation ability among E. coli strains. Firstly, 7 strains of Avian pathogenic E. coli were detected using irp2 PCR amplification, ERIC-PCR genotyping of the strains was conducted, and the strains were divided into two types; secondly, 17 strains were detected and the results showed that the positive percentages of irp2, irp3, irp4 and irp5 were 41.2%, 64.7%, 29.4% and 35.3%, respectively; finally, the biofilm formation ability of the isolated strains of E. coli were determined and the result showed that the positive strains carrying irp2 have a strong ability of biofilm formation. The results suggested