化痰活血方对高脂血症大鼠降脂作用及其机理的实验研究-中医临床基础专业论文.docxVIP

化痰活血方对高脂血症大鼠降脂作用及其机理的实验研究 化痰活血方对高脂血症大鼠降脂作用及其机理的实验研究 博士学位论文 Abstract Ob iect i ves：Based on the summary and analysis of the entrent documents，the study explores roles of phlegm and stasis in the formation of Hyperlipidemia．By analyzing the influence of the Huatan Huoxue Fang(HHF’on expedmental Hyperlipidemia in rats and on bovine aorta endothelial cells(BAEC)，the author goes further to sort out the efrect of the HHF in the treatment of Hyperlipidemia and its working mechanism． Methods：Theoretical Inquiry：The methods of documents collection，summary, and analysis are adopted．Experimental Inquiry：A．The influence on the experimental Hypempidemia in rats，the rats are sub．divided randomly into the normal control group，the model group，Fenobrite group，and finally,big，middle，and small groups according to HH T．1．Use enzyme methods and immunization photoextinction individually to detect the Ievels of total cholesterol(TC)，triglyccridc(TG)，low density lipoprotein cholesterol(LDL—c)，hi曲deasity lipopmtein cholesterol(HDL-c)， HDL2-c，，apolipoprotein A(ApoAI)and apolipoprotein B(ApoBtoo)}in blood，use 2％ -l 6％non-denaturing poplyacrylamide gradient gel electrophoresis to detect the plasma level of small dense low—density lipoprotein(sdLDL)．2．111e observation of the improve of hemorheology by testing whole blood viscosity,plasma viscosity and red-cell specific volume；3．Use enzyme methods to detect superoxide dismutase (SOD)，malondialdehyde(MDA)and nitric oxide(NO)content．4．Use optical microscopes to monitor the morphologic change of liver． 5． Use immunohistochemistry assay to detect the expressed level of low density lipoprotein receptor(LDL-R)in liver．6．Use Rr-PCR t0 get the levels of low density lipoprotein receptor mRNA(LDL—RmRNA)．peroxisome proliferators actovated receptor alpha mRNA (PPARamRNA)and acyl—CoA oxidase mRNA rACOmRNA)in liver．B．Tl坞 influence on BAEC．the serum containing HHF ofdifierent concentration(5％、10％ 、20％)add to the 3-5 era BAEC，10 hours after applying Westem Blo