高产麦芽低聚糖酒曲根霉的选育及其主要淀粉降解产物研究-食品科学专业论文.docxVIP

Studies Studies On Selection and Cultivation of High Maltooliga— saccharides Producing Rhizopus in Distillers’Yeast and ItsMajor Degrading Products from Starch Abstract 鼬豇叩淞is mainly used in fermentation for its enrichment amylases血me glutinous rice wiIle it the IIlaill IIlicrobe of saccharification．To enhance the functional oligosaccharides in tlle西udno— rice wine．This experiment studied the amylases of Rhizopus and the relation wimⅡle malt一 00hga8accharides content,selected and cultivated the strain that highly yield maltooligosacch折 ides，studied the characters of the glucoses．This thesis deals with five Darts= 1 Thi8 experiment made selection and purification of Rhizopus from DistiⅡers，yeast．We gained fow 8妇iIl8 Rhizopus RN一1，Rhizopus RA一1，Rhizopus RS．1 and足她rop凇RQ一1 which had oligosacc晡dase activity,these four strains produce a—amylase and glucoamylase bener tilaIl 咖ditional Q303·The glutinous rice wine made with R^弦叩Hs RA．1 has Ⅱle most maltooligosaccharides． 2 we gained励却船RA—Uv4 with 403．75 higher oligosacchridase，486．2％h遮her Q—amylase， 567·8％higher glucoamylase than the original after mutagenesis by ul妇violet and N扪，on Rhizopus RA一1． 3 Amylases of Rhizopus RA—UV4 separated and purified by(NH4)2S04 fractional precipitation，DEAE—cellulose ion—exchange chromatography and gel G一1 00 filtration． n was cIe蜥nedby PAGE，the amylase shows glucoamylase line and two Q一锄ylase lines，ttle three molecular weights detemined by SDS—PAGE are about 78 kD，48 kD and 50 kD． 4 We made glutinous rice wine with Rhizopus RA—UV4．After the gel G一100 colIlmn we f；Dund 烈u∞8e、di88accharide、triasaccharide．The glutinous rice wine yield total sugar 355．43啦 g(rice),reducing sugar 305。21 g／kg(rice)，oHgosaccharides value 14．13％，total ohgosacch撕des 50．21 g／kg(rice)，105·9％higher than the original triasaccharides 25．43蹴∞ce)，1 19．1％ higher than the original Ke)，、Ⅳords：glutinous rice Wine，Rhizopus，maltooligosaccharides，amylase：cultivation Ⅱ 独创性声明与关于论文使用授权的说明独创性声明 独创性声明与关于论文使用授权的说明 独创性声明 本