《盐胁迫下骆驼刺组织中钠离子和氯离子的测定》-毕业设计论文（学术).docVIP

毕业论文 PAGE 目 录 摘要································································1 关键词······························································1 前言································································2 1.材料与方法·······················································3 1.1 试验材料·······················································3 1.1.1 植物样品···················································3 1.1.2 试剂·······················································3 1.1.3 主要仪器····················································3 1.2 试验方法·······················································3 1.2.1 试验材料的准备·············································3 1.2.2植物样品的前处理············································4 1.2.3测定方法····················································4 1.2.4 标准曲线的绘制·············································5 2 .结果与分析······················································5 2.1 样品液测定·····················································5 2.1.1 骆驼刺中氯离子的测定········································5 2.1.2 骆驼刺中钠离子的测定········································6 2.2 标准曲线的分析·················································7 2.3 实验数据的重复性分析···········································7 2.4 盐胁迫对骆驼刺体内Na+和Cl_含量影响·······························7 4结论·····························································9 谢 辞····························································10 参考文献 ·························································11 盐胁迫下骆驼刺组织中钠离子和氯离子的测定 杜曼·加勒卡森 摘要：针对骆驼刺在西北干旱地区绿化中的使用, 本论文用0、50、100、200mM/L NaCl溶液分别对骆驼刺种子进行胁迫处理, 在人工气候箱内温度25℃，光照强20001x的条件下无菌培养0、5、10、15天的骆驼刺用碳酸钠－碳酸氢钠淋洗液过夜处理后，用电位滴定法和离子选择电极法分别测定了组织中钠和氯的含量。研究了骆驼刺体内Na+和Cl-的含量的变化与盐胁迫的关系。结果表明:随着NaCl胁迫时间和浓度的增加,骆驼刺中Na+、Cl-的含量增加，这也骆驼刺正说明了具有一定的耐盐能力。当盐浓度达到一定程度时，Na+、Cl-含量开始下降，表明随着盐分浓度的升高，对细胞膜产生伤害。骆驼刺种子发芽、幼苗生长（培养时间为25天）能忍耐200 mM/L的盐液（NaCl）胁迫。 关键词：骆驼刺 ；耐盐性 ； 盐胁迫 ；钠离子 ；氯离子 前言 骆驼刺(Alhagi sparsifolia)为豆科(Leguminosae)蝶形花亚科( Pap ilionatae)骆驼刺(Alhagi Gagneb. )的半灌木，该植物广泛分布在亚欧大陆的干旱或半干旱地区及非洲部分地区。在我国主要分布在大西北