赶黄草提取物对胆汁淤积性肝细胞损伤的保护作用-外科学（小儿外科）专业论文.docxVIP

构较完整，小叶间胆管基本正常，汇管区有少量炎症细胞浸润，肝细胞形 构较完整，小叶间胆管基本正常，汇管区有少量炎症细胞浸润，肝细胞形 态正常，部分肿胀，个别呈空泡样变性。肝组织损伤程度较组2明显减轻。 3．电镜病理学结果：组1术后7d：相邻的肝细胞排列整齐，分界清楚， 结构正常，细胞核大而圆，核仁清晰而明显，染色质分布正常，核膜 结构清晰，线粒体结构正常，嵴结构清晰，内质网结构清楚，排列规 则，胞质内富含糖原，染色均匀。组1术后1 4d与术后7d肝细胞超微 结构无明显变化。组2术后7d：部分细胞明显水肿，胞内出现大小不 等脂滴，细胞核边聚，核大，异染色质增多，核膜皱缩，线粒体肿胀， 部分嵴断裂，核周隙增宽。术后14d：肝细胞明显水肿，胞内大量脂滴， 细胞核呈现边集征象，核膜皱缩，线粒体明显肿胀，部分嵴断裂， 呈空泡样变。组3、组4术后7d、1 4d：细胞结构基本正常，线粒体 稍水肿、内质网扩张，损伤较轻。两组间肝细胞超微结构无明显差异。 4．脂质过氧化指标结果：组2术后7d、14d MDA、ROS持续升高，明显 高于组1(PO．05)，组3、组4术后7d、14d MDA、ROS均低于组2(P0．05)， 两组间MDA、ROS无明显差异(PO．05)。组2术后7d、14d SOD降低， 明显低于组1(尸O．05)，组3、组4术后7d、14dSOD均高于组2(尸O．05)， 两组间SOD无明显差异(尸O．05)。结论：1．赶黄草提取物能降低胆汁 淤积性肝损伤大鼠肝功能酶学指标，减轻淤胆性黄疸，维护线粒体形态结 构和功能，减轻氧化应激反应对细胞的损伤，对胆汁淤积性肝细胞损害起 到保护作用。2．其机制可能与减轻氧化应激导致的脂质过氧化损害有关。 关键词：肝细胞；损伤；赶黄草；保护 万方数据 The The protect effect of the extracts from Penthorum Chinese Pursh on cholestatic hepatocyte damage Abstract objectives：To establish the model of cholestatic hepatocyte damage in rats by common bile duct ligation and build a randomized controlled trial，in whi ch experimental rats were given the extracts from Penthorum chinense Pursh or ursodeoxycholic acid by gavage．To observe the changes of liver function and lipid peroxidation index by serological tests．The histopathology and ultrastructure of hepatocytes in the model animals were observed by light microscopy and transmission electron microscopy．The protective effects of the extracts from Penthorum chinense Pursh for cholestatic hepatocyte damage were investigated and the mechanism was discussed through lipid peroxidation of oxidative stress pathway． Methods：1．Establishing cholestatic hepatocyte damage rat models：Sprague —Dawley rats were raised in the SPF asepsis animal room and were adapted to the environment after 7 days．2％pentobarbital(1 00mg／kg)was intraperitoneally injected to anesthetize the rat．The common bile duct was revealed through laparotomy．The common bile duct was double ligated and transected between the two lines．2．Groups：1 5 normal male