高山红景天中的红景天甙、甙元酪醇、香豆素的生产工艺-植物学专业论文.docxVIP

摘 摘 要 本项研究应用先进的技术，对高山红景天的有效成分红景天甙、甙元酪醇、香豆索 的生产工艺路线进行改革，经过大量的探索和尝试．总结出一条经济高效的工艺路线， 不仅提高了生产技术水平和质量控制水平，而且得到高质量、高附加值的高山红景天系 列产物。 研究依据高山红景天中各成分的性质差异，应用超临界co：萃取技术、大孔树脂吸 附纯化技术、高效液相色谱分析等先进的提取、纯化及分析技术，分别对各有效成分提 取、纯化、分析的工艺条件进行研究探讨，得出一套完整高效的工艺路线，实现了高山 红景天中有效成分香豆紊、红景天甙、甙元酪醇的有效分离，产物中红景天甙和甙元酪 醇含量达80％以上，共收率达0．35％；香豆素含量达24％，收率达896。以上三个产物的纯 度和收率均达到产业化的最高水平，该研究成果为高山红景天类药物形成系列产品，开 发新药奠定了基础。 ， ￡该课题研究所得的高山红景天系列产品的生产工艺，现已经成功通过中试放大，中 试结果进一步证实该生产工艺是确实可行、经济高效的，所得产物附合中药现代化对中 药产品提出的质量要求，该研究成果有很广阔的应用前景／7， 关键词：高山红景天；超临界二氧化碳萃取；树脂吸附；红景天甙：甙元酪醇；香豆素 高效液相 AbstractWe Abstract We used the advanced techniques to reform the production process for the effeetive constituents of Zho出ola sachalinensis：eoumarin．salidrOSide and p-tyros01．After lots of tests，we acquired an economical and high efficient technological route that not only increased the technological level to produce and the level to control the quality but alSO gained serial products with high quality and high added value． According to quality difference of different constituents of肋。出ola sachalinensis,we used advanced techniques such aS extraction technique of supercritical carbon dioxide．purification technique of resinous adsorption and analysiS teehnique of HPLC to find out technological tenditions of extraction， purification，and analysis．We acquired a complete and high efficient technological route and implemented effective separation of effective constituents of Rhodiola sachaiinenMs：coumarin．sal tdrostde and P—tyros01．The content of salidroside and p—tyros01 in the product could reach more than 80％ and the total yield efficiency was 0．35％：the content of coumarjU in the product reached 24％and the yield efficiency was 8％．The putity and Yield efficiency reached the highest level of mass—production．This research achievoment offered a basis for RhodloIa sachaltnensis medieine to form serial products and for new medicine to be developed．The production process of serial products of RhodioIa saeha]抽ensls based on this research work has bee