高基质利用率和高生物活性成分灵芝杂交菌株育种-食品科学与工程专业论文.docxVIP

万方数据 上海海洋大学硕士学位论文 Study on breeding of Ganoderma lucidum Hybrid Strain with High Substrate Utilization Rate and High Biological Activity Components ABSTRACT Ganoderma lucidum is a treasure in our country’s medicine. For long it has been called “fairy herb” or “lucky herb”. The spore of Ganoderma lucidum is difficult to germinate in the artificial condition. It is unable to obtain mononuclear strain for Ganoderma lucidum breeding easily, which make conventional methods of breeding to develop slowly. In the study, the hybrid strains of Ganoderma lucidum was obtained succeessfully through protoplasting Monokaryotization technology, the results showed that Hybrid Strain with High Substrate Utilization Rate and High Biological Activity Components were selected by liquid fermentation test. The mycelial growth rate, laccase relative activity, CMCase relative activity of twenty-seven strains were measured, and the yield of fruiting body was obtained by cultivation. The correlations between mycelial growth rate and yield, laccase relative activity and yield, CMCase relative activity and yield were established. To further understand relationship of thefactors with yield of fruiting body, Pearson correlation analysis was used, mycelial growth rate, laccase relative activity and CMCase relative activity was demonstrated high correlation with yield. The results showed that the correlation between these indexes and yield were positively linear. The yield could be forecasted according to these indexes without cultivation. The model of selecting parent strains for Ganoderma lucidum hybridization breeding was estabilished, which was handy and timesaving. G0119, G0130 and G0154 were chosen as parent strains. 108 protoplast regenerating mononuclear strains were obtained through monokaryotization by protoplasting technique, and six different mating type was found in these mononuclear strains. By hybrid breeding 63 hybrid strains which are different from parents were obtained. Inadditi