红参中外源糖含量的测定_张浩.pdfVIP

56 Special Wild Economic Animal and Plant Research 特 产 研 究 文章编号：1001- 4721（2011）04- 0056- 04 红参中外 源 糖 含 量 的 测 定 1 1 1 2 1※ 张浩 ，许世泉 ，张瑞 ，陈少麟 ，王英平 （1.中国农业科学院特产研究所，吉林 吉林 132109；2.抚松县人参研究所，吉林抚松 134500） 摘要：通过对紫外吸收光谱、试剂用量、显色温度等因素进行考查，确定苯酚- 硫酸法测定红参单寡糖含量的最佳试验条件。结 果表明，5%苯酚1mL、硫酸7mL、60℃保温30min、检测波长490nm 时，葡萄糖检测浓度在20~120μg/mL 范围内与吸光度呈良 2 好的线性关系（R =0.999 3），平均回收率100.3%，RSD=2.99% 。该方法操作简便，精密度、重现性、稳定性良好，测得加糖红参中 单寡糖含量是未加糖红参（对照）的2~4 倍，可用于红参外源糖的检测。 关键词：苯酚- 硫酸；红参；多糖 中图分类号：R284.1 文献标识码：A Study on Detection of Exogenous Sugars of Red Ginseng 1 1 1 2 1※ ZHANG Hao ，XU Shi- quan ，ZHANG Rui ，CHEN Shao- lin ，WANG Ying- ping （1.Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Jilin 132109, China; 2. Fusong Institute of Ginseng, Fusong 134500, China） Abstract ：In order to determine the optimum testing conditions, the wave length, reagents and reaction temperature etc were investigated. Results showed that when the 1.0mL of 5% phenol aqueous solution and 7.0mL of concentrated sulfuric acid 2 were used to complete the reaction at 60 ℃, glucose had a good liner relationship (R =0.993) with absorbency ranged from 20 to 120μg/mL, and with an average recovery rate of 100.3%, RSD= 2.99%. Total content of oligosaccharides in red ginseng (added sugar) was 2~4 times higher than CK. This method, with good precision, reproducibility and stability, was simple and convenient, and was suitable for detection of red ginseng foreign sugar. Key words ：Phenol- sulfuric acid; Red ginseng; Polysaccharide 红参为五加科植物人参（panax