环介导等温扩增技术快速检测双歧杆菌属细菌-食品安全质量检测学报.PDF

7 3 Vol. 7 No. 3 2016 3 Journal of Food Safety and Quality Mar. , 2016 范一灵, 杨美成* ( , 201203) 摘 要: 目的 (LAMP)方法 16S rDNA LAMP , 结果 5 19 , 100%60 min , DNA 16.1±8.2 pg/μL, 0.1 CFU/mL结论 LAMP , DNA 关键词: ; ; ; 16S rDNA Rapid detection of Bifidobacterium spp. by loop-mediated isothermal amplification * FAN Yi-Ling, YANG Mei-Cheng (Shanghai Institute for Food and Drug Control, Shanghai 201203, China) ABSTRACT: Objective To develop a specific and rapid method for detection of Bifidobacterium spp. by loop-mediated isothermal amplification (LAMP). Methods Specific DNA fraction of 16S rDNA gene of Bifidobacterium spp. was targeted for primer design of LAMP. Bifidobacterium spp. was confirmed by the changes of color or turbidity of reactions specifically. Results Five strains of Bifidobacterium spp. and 19 strains of non-Bifidobacterium spp. were tested respectively in 60 min. The specificity and exclusivity of the method were 100%. The detection limit of DNA from Bifidobacterium spp. was 16.1±8.2 pg/μL, and the detection limit of thallus from