基于葡萄糖氧化酶介孔碳复合材料葡萄糖生物传感器.pdfVIP

Journal of Sensor Technology and Application 传感器技术与应用, 2015, 3, 69-78 Published Online July 2015 in Hans. /journal/jsta /10.12677/jsta.2015.33009 Glucose Biosensor Based on Glucose Oxidase/Ordered Mesoporous Carbon Composite Material Yue Tang, Qinghe Wei, Yujia Liang, Yan Ji, Bin Qi* Faculty of Chemistry, Northeast Normal University, Changchun Jilin * Email: qib219@ nd th th Received: Jun. 22 , 2015; accepted: Jul. 10 , 2015; published: Jul. 16 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Glucose oxidase (GOD) was adsorbed on the surface of OMC modified glassy carbon electrode (GOD/OMC/GCE). Then ferrocene was assembled on the GOD/OMC/GCE platform to construct an amperometric glucose biosensor. The results showed that the Fc/GOD/OMC nanocomposite exhi- bits a remarkably improved catalytic activity towards the oxidation of glucose. The biosensor ex- hibited a wide linear response up to 10 mM with a low detection limit of 1.03 ± 0.15 μM (S/N = 3). The selectivity and stability of the biosensor were also investigated. Keywords Ordered Mesoporous Carbon, GOD, Glocuse, Biosensors 基于葡萄糖氧化酶/介孔碳复合材料的葡萄糖生 物传感器 唐 悦，魏倾鹤，梁羽佳，吉 艳，齐 斌* 东北师范大学化学学院，吉林 长春 * Email: qib219@ *通讯作者。 69 基于葡萄糖氧化酶/介孔碳复合材料的葡萄糖生物传感器 收稿日期：2015年6月22 日；录用日期：2015年7月10 日；发布日期：2015年7月16 日 摘 要 我们采用浸渍法将GOD吸附在有序介孔碳修饰的电极表面(GOD/OMC/GCE)，然后又在其表面覆盖了一 层二茂铁丙酮溶液，最终成功构建了一种新的电流型葡萄糖生物传感器。结果表明，该修饰电极 Fc/GOD/OMC/GCE对葡萄糖有很好的电催化活性，对葡萄糖的检测线性范围为5.00 μM~10.00 mM；检 出限为1.03 ± 0.15 μM (信噪比为3)。同时对该修