农杆菌介导玉米幼胚愈伤组织遗传转化体系优化.pdfVIP

DOI :10.13432/j.cnki .jgsau.2010.06.005 2010 12 　　　　　　　 4 5 6 49～54 JOURNAL OF GANSU AGRICULTURAL UNIVERSIT Y 庄志扬, 王汉宁, 张金文, 温睿婷,李永生,郑琪 (, 　730070) :`58, , ., (AS) 150 mg ·L-1 , 17.39%;D600 0.3、 20 min、60 h、22 ℃、 H 5.4 400 mg ·L-1 , , 17.26 %. :;;; :S 513　　　　:A 　　　　　:1003-4315(2010)06-0049-06 O timization of genetic transformation system for maize immature embryo calli mediated by A g robacteri um tumef aciens ZH UA NG Zhi-yang,WANG Han-ning,ZHANG Jin-w en,W EN Rui-ting, LI Yong-sheng,ZHENG qi (College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China) Abstract:The immature embryo of excellent maize inbred “Zheng-58”w as used in the ex eriment, tak- ing the embryogenic callus as the rece tor for the transformation of Trivalent lant ex ression vector w hich w as mediated by A gr obacter ium t umef aciens .Im ortant factors w hich influenced the efficiency of the transformation w ere identified and an efficient transformation system w as develo ed for maize immature embryo calli.The results show ed that the resistant calli rate w as remarkably im roved (17.39 %higher than other concentration)w hen the concentration of acetosyringone (AS)w as 150 mg ·L-1 both in the in- fection solution and co-culture medium .A higher efficiency system for transformation w as established w ith the combination of 0.3 D ×20 minutes for A .t umef aciens concentration and incubation-time,20 ℃×60 h for the co-culture tem erature and time, 400 mg ·L-1 for the cefotaxime (Cef)concentration,and the H w as 5.4.By this o timized system, the rate of the resistant calli increased by 17.26 %. Key words:maize;immature embryo calli;Ag robacter i um t umef a