解钾菌的筛选.pdf

27 3 () Vo.l27　No.3 2009 9 JournalofHubeiUniversityforNationalities(NaturalScienceEdition) Sep.2009 (湖北民族学院生物科学与技术学院 湖北恩施 44 5000 ) :以钾长石粉为惟一钾源的硅酸盐细菌选择 培养基筛选能够降解土壤中钾元素的细菌.利用梯度稀释 分离法和平板划线分离法对其中的细菌进行筛选 用火焰分光光度法测定培养液和细菌细胞内的钾含量 以筛选 出高效菌株.结果表明:dk8、dk7、 dk14在解钾培养基上生长状况良好 以 dk8分解钾长石的能力最强 可作为制作 微生物肥料备选用菌. :解钾菌;钾长石;速效钾 :Q939.9 :A :1008-8423 (2009 )03-0285-04 SeparationandPurificationofthePotassium-releasingBacteria ZHOUY-ifengLUOYun-xiaLIUHua-zhong (SchoolofBiologicalScienceandTechnologyHubeiUniversityforNationalitiesEnshi445000 China) Abstrac:tInthispaperweseparatedandpurificatedthebacteriaforreleasinginsolublepotassiumfrom soilbytheselectivemedium inwhichpotashfeldsparpowerwastreatedastheonlysourceofpotassium. Wedilutedthebacteriawithgradientandlinedoncultureplateforseparationandpurificationofthebac- teriaforreleasepotassium andusedflamespectrophotometerfortestspotassiumcontentinnutrientsolu- tionandthecellofbacteriaandthenselsctedhigh-efficiencybacteria.Theresultsshowthatdk7 dk8 anddk14 straincangrowwellontheselectiveplatedk8 isthebestoneforreleaseavailablepotassium frompotashfeldsparpowe.rThereforedk8 canbeusedascandidatestrainforproductionofmicrobial fertilize.r Keywords:potassium-releasingbacteria;potashfeldspa;ravailablepotassium [1] [2] 60 、 、、. 60% (2 ～3 )×10-6 、、 [3] 3 . [4] 、 、、. [5] 95% 2%. [6] 、 . 、 、、、 :2009 -06-20. : ()(200725); . : (1978- )() . 286