无机优先污染物与DNA之间作用的传感研究.docVIP

无机优先污染物与DNA之间作用的传感研究 PAGE 无机优先污染物与DNA之间作用的传感研究 第 PAGE 1 页 共 27页 无机优先污染物与DNA之间作用的传感研究 摘要 本文通过研究，采用石墨烯-ZnS量子点这种新型纳米材料修饰玻碳电极，由于其具有大的比表面积、高的载流子迁移率、良好的导电导热性、优异的机械柔性和化学稳定性，从而可以促进DNA与电极之间的电子转移；以循环方波伏安法为测定方法，以HAc-NaAc缓冲溶液为电解质溶液，考察了GO-ZnS/GCE复合电极在含有污染物Cr6+的DNA溶液中的电化学响应，实验结果表明：Cr6+的加入降低了DNA在0.7V、1.0V左右的氧化峰，该峰分别是腺嘌呤与鸟嘌呤的氧化峰，发现Cr6+浓度在1.0×10-8mol/L~1.0×10-2mol/L时，峰降低的幅度ΔI与Cr6+的浓度对数呈良好的线性关系，线性方程为：y=4.95045x+0.5025LgCCr6+，线性相关系数为0.99875，该电极具有较好的稳定性与重现性。 关键词：石墨烯-ZnS量子点；循环方波伏安法；DNA；Cr6+ SENSING STUDY INTERACTIONS BETWEEN INORGANIC PRIORITY POLLUTANTS AND DNA ABSTRACT ???This paper studies the use of graphene-ZnS quantum dots This new nano-modified glassy carbon electrode material, because of its large surface area, high carrier mobility, good electrical and thermal conductivity, excellent mechanical flexibility and chemical stability resistance, which can facilitate electron transfer between DNA and the electrode; to cycle square wave voltammetry method for the determination to HAc-NaAc buffer solution as the electrolyte solution was investigated GO-ZnS/GCE composite electrode containing contaminants of Cr6 + the electrochemical response of the DNA solution, the experiment results show that: Cr6 + to decrease in the DNA in the 0.7V, 1.0V oxidation peak around the peak of the oxidation peaks of adenine and guanine, the concentration of Cr6 + was found at 1.0 × 10 - 8mol / L ~ 1.0 × 10-2mol / L, the peak amplitude ΔI with lower concentrations of Cr6 + logarithmic relationship was linear, linear equation: y = 4.95045x +0.5025 LgCCr6 +, the linear correlation coefficient of 0.99875, the electrode having a good stability and reproducibility. ???? Key words: graphene-ZnS quantum dots; cyclic square wave voltammetry; DNA; Cr6+ 目 录 1 绪论………………………………..………………………………………………….……1 1.1课题研究的背景 …………………….……………………………………………1 1.2 六价铬的检测方法方法………………….…………………………………………. 1 1.2.1 分光光度法……………..…………..…………………………………………. 1 1.2.2 原子吸收法……………………………………