HaNPV病毒对寄主昆虫的持续作用研究-林业科学研究.PDFVIP

2006, 19( 2): 2 16~ 220 ForestResearch : 100 11498( 2006) HaNPV 1 1* 1 2 曲良建 , 张永安 , 王玉珠, 郎杏茹 ( 1. , , 100091; 2. , 750001 ) : H aNPV 3 , , , , PCR, , : ; ; PCR : S763 : A Studies on Sustainable Effects forH elicoverp a arm ig era Nuclear Polyhedrosis Virus to Host Insects 1 1 1 2 QULiangjian, ZHANG Yongan, WANG Yuzhu , LANGX ingru ( 1. Resear h Institute o fForest E o logy, Environm ent and Prote tion, CAF, Key Laboratory of Forestry Prote tion, State Forestry Adm inistration , Beijing 100091, China; 2. Center Station of Contro l andQ uarantine of Forest D iseases and Pests, Y in huan 750001, N ingx ia, China) Abstract: Third instars larvae of otton bolwl orm, H elicoverpa ar igera, w ere infe ted w ith different on entrations ofH eli coverpa ar igera nu leopolyhedrov irus(H aNPV). Survivorswere o lle ted respe tively and reared in laboratory room. M or tality rate of the larvae, averagew eight of the single pupa and pupation ratew ere observed in parental generation and su essive filial generations. T he results show ed that the v irus not on ly led to death of larvae in parental and folialgeneration, but also mi pa ted the averagewe ight of the single pupa and pupation rate, espe ially to the parental and the first fo lial gen eration. T he expermi ental data ind i ated that the virus ould weaken the host in folial generations, wh i h ould ontrol popu lations of the host for a long tmi e. M eanwhile, polymerase ha in rea tion ( PCR) amplifi ation of po lyhedrin gene se quen es demonstrated that the viruswas present in eggs and pupaewhose parentswere in