中国芒Miscanthussinensis种质资源SSR标记遗传多样性分析.PDF

植物遗传资源学报2013,14(1):　 -　 Journal of Plant Genetic Resources 中国芒(Miscanthus sinensis)种质资源 SSR标记 遗传多样性分析 肖　 亮,薛　 德,蒋建雄,艾　 辛,覃静萍,陈智勇,刘树玲,易自力 (湖南农业大学生物科学技术学院,长沙410128) 　 　 摘要:利用33对SSR 引物对来自中国16 个省的46份野生芒(Miscanthus sinensis)种质进行遗传多样性分析。 结果显示: (1)33对SSR 引物共扩增出87条DNA条带,75条为多态性条带,占86．21%,条带大小范围80~310 bp;(2)遗传多样性参数 分析结果:Shannon′s 信息指数(I)变幅为0．020 ~1．522,平均0．745,引物多态性信息含量(PIC)变幅为0．040 ~0．738,平均 0．445,遗传相似系数(GS)的变幅为0．315~0．933,平均0．569,说明我国芒种质资源遗传基础宽,遗传多样性丰富;(3)相似 系数UMPGA聚类结果与主成分分析(PCA)结果一致,可将46份种质分为3 大类群,类群 Ⅰ主要由中部芒组成,类群Ⅱ主要 由北方芒组成,类群Ⅲ主要由南方芒组成,西南芒在每个类群中均有渗透,这一结果说明芒种质资源的遗传分化与其种源的 地理分布有一定的相关性,但与地理起源不能完全吻合。 　 　 关键词:芒;种质资源;SSR标记;遗传多样性 Genetic Diversity inMiscanthus sinensis of China Revealed by Simple Sequence Repeats DNA Markers XIAO Liang,XUE De,JIANGJian-xiong,AI Xin,QINJing-ping,CHEN Zhi-yong,LIU Shu-ling,YI Zi-li (College of Bioscience Biotechnology,HunanAgricultural University,Changsha410128) Abstract:In order to study the genetic diversity ofMiscanthus sinensis from China,46 collectionsofM.sinensis from 16 provinceswereanalyzedby the simple sequencerepeats(SSR)markers.Theresultsshow that33 SSRprim- er pairs produced 87 bandswith75 polymorphic locis(86．21%)and the size of amplified DNA varied from 80 to 310bp.Shannon diversity index(I)is0．020-1．522,with a mean of0．745.Polymorphic information content(PIC)is about0．040-0．738,with a mean of 0．445. Genetic similarity coefficient is about 0．315-0．933,with a mean of 0．569.UPGMA and PCA Cluster analysis indicated that46 collections of M.sinensis were clustered into 3 groups. Group I was mainly composed of the germplasm from north China,group II composed of those from center China, and group III composed of those from south China.But some collections from southwest of China were divided into the above three groups.These results indicated that there was some relatio