树脂吸附法分离瓜果腐霉毒素中除草活性物质.PDFVIP

微生物学通报 SEP 20, 2008, 35(9): 1505~1509 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 生物实验室 树脂吸附法分离瓜果腐霉毒素中的除草活性物质 张利辉 王毅靖 张金林 董金皋* ( 071001) 摘 要: 本试验以对禾本科杂草具有除草活性的瓜果腐霉菌株 PAM1 为试验材料, 研究了 X-5 、 AB-8 、H-103 和 S-8 等4 种大孔吸附树脂对其除草活性物质的吸附性能, 结果表明X-5 树脂能够 较好地吸附该除草活性物质。选用 50% 乙醇、95% 乙醇、乙酸乙酯和丙酮4 种溶剂为供试洗脱溶 剂, 采用 2 种洗脱方案, 对其最佳洗脱溶剂进行了选择, 生长抑制作用和种子萌发试验结果表明, 以方案1 洗脱为最佳。 关键词: 瓜果腐霉病菌, 除草活性, 树脂柱层析 Colophony Adsorbing Isolating Herbicidal Substance from Mycotoxin Produced by Pythium aphanidermatum * ZHANG Li-Hui WANG Yi-Jing ZHANG Jin-Lin DONG Jin-Gao (College of Plant Protection of Agricultural University of Hebei, Baoding 071001) Abstract: Four kinds of colophony were used to isolate the herbicidal substance from the toxin produced by isolate PAM1 of Pythium aphanidermatum, which had herbicidal activity to Digtaria sanguinealis L and the results showed X-5 was the best one for absorbing herbicidal substance among the four kinds of colophony used. Four solvents including 50% ethanol, 95% ethanol, ethyl acetate and acetone were used as eluting sol- vent and 2 procedures were tested, and the results of growth inhibition and seed germination indicated that the best eluting procedure was procedure 1. Keywords: Pythium aphanidermatum, Herbicidal activity, Colophony , , 10~25 [1] , [3] , , , ,