产纤维素酶的金橙黄微杆菌YT9分离鉴定及其培养条件优化.PDF

210 2013, Vol.34, No.17 食品科学 ※生物工程 产纤维素酶的金橙黄微杆菌YT9分离鉴定及其 培养条件优化 * 缪 静，李坦坦，程仕伟 ，梁会会，崔海洋 (鲁东大学生命科学学院应用微生物研究所，山东 烟台 264025) 摘  要：从渤海近海土壤中分离1株产纤维素酶菌株，经形态、生理生化指标和16S rDNA分析鉴定为金橙黄微杆 菌。对该产酶菌株进行碳源、氮源、金属盐、培养温度、pH值和诱导物质量浓度的优化，确定最佳培养基组分 和条件为：果糖20g/L 、羧甲基纤维素钠(CMC) 0.5g/L 、蛋白胨8g/L 、硫酸亚铁铵2g/L 、K HPO 2g/L 、NaH PO 2 4 2 4 2g/L 、MnSO4 1g/L 、pH6.5 、培养温度30 ℃、接种量4% 。优化后纤维素酶系中以羧甲基纤维素酶活力为最高，为 β 226.27U/mL ，其次是 -葡萄糖苷酶和微晶纤维素酶。 关键词：金橙黄微杆菌；纤维素酶；培养条件优化；分离；鉴定 Isolation, Identification and Culture Characteristics of Cellulase-producing Exiguobacterium sp. YT9 MIAO Jing，LI Tan-tan ，CHENG Shi-wei∗，LIANG Hui-hui ，CUI Hai-yang (Institute of Applied Microbiology, College of Life Sciences, Ludong University, Yantai 264025, China) Abstract ：Strain YT9 with high-yield cellulase was screened from coastal soil samples, and the morphological, physiological and biochemical characteristics as well as 16S rDNA sequence homology of the selected strain were studied . Finally , the strain was identified as Exiguobacterium sp . Meanwhile, medium composition and culture conditions for cellulase production were optimized. The optimal medium for higher production of cellulase consisted of 20 g/L glucose, 0.5 g/L CMC, 8 g/L tryptone, 2 g/L ferrous alum, 2 g/L K HPO , 2 g/L NaH PO , and 1 g/L MnSO . 2 4 2 4 4 The maximum activity of CMCase was 226.27 U/mL at pH 6.5, 30 ℃ and 4% inoculum. At the same time, the activities of