基于磁泳的生物分离分析技术3.PDFVIP

第 18 卷 第 23 期 化　学　进　展 Vol. 18 No. 23 2006 年 3 月 PROGRESS IN CHEMISTRY 　Mar. , 2006 基于磁泳的生物分离分析技术 车津晶　万谦宏 (天津大学药物科学与技术学院 　天津 300072) 摘 　要 　利用磁场诱导的微粒运动即磁泳对磁响应性粒子进行精细分离 ,是近年来发展起来的选择性 分离细胞和高分子量核酸的有效技术。本文在阐明磁泳分离原理的基础上 ,介绍了磁泳分离的分流薄层分 级技术、四极磁场流动分离技术和微芯片上的自由流磁泳分离技术的装置构造、工作原理及其在生物分离分 析中的应用。 关键词 　磁泳 　分流薄层分级 　四极磁场流动分离 　微芯片自由流分离 　生物分离 ( ) 中图分类号: O6572 ; O6589 　文献标识码 : A 　文章编号 : 1005281X 2006 023034405 Bioseparation and Bioanalysis Based on Magnetophoresis Che jinjing 　Wan Qianhong (College of Pharmaceuticals and Biotechnology , Tianjin University , Tianjin 300072 , China) Abstract 　Magnetically induced migration of particles in liquid , also known as magnetophoresis , is an effective approach for selective separation of bioparticles such as cells and large nucleic acids. The principle of magnetophoresis is introduced based on the balance between magnetic and drag forces acting on a magnetically responsible particles in liquid. Three magnetophoretic separation methods including magnetic splitflow thin fractionation , quadrupole magnetic flow sorting and onchip freeflow fractionation are described with emphasis on device structure , operation principle and application in bioanalysis and bioseparation. Key words 　magnetophoresis ; splitflow thin fractionation ; quadrupole magnetic flow sorter ; onchip freeflow fractionation ; bioseparatioin 发展起来的高效、高通量生物分离分析技术 ,在细胞 1 　引 　言 生物学、生物技术、生物医学、临床研究等领域中用 在生物体中大部分分