乳酸细菌胞外多糖研究.docVIP

PAGE 10 PAGE 10 LANZHOU UNIVERSITY OF TECHNOLOGY 毕业论文 题 目 学生姓名 学 号 专业班级 指导教师 学 院 答辩日期 第 页 第 页 PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT 1 乳酸细菌胞外多糖的研究 摘要 乳酸细菌(LAB)胞外多糖（EPS）是乳酸细菌在生长代谢过程中分泌到细胞壁外的的荚膜多糖和粘液多糖的总称。本实验采用酶解法从乳酸细菌发酵液中提取胞外多糖。通过测定胞外多糖的含量筛选出了2株高产胞外多糖的菌株，并运用单因素及正交实验对其发酵条件进行优化，优化后的最佳发酵条件是：初始培养基pH5.5，乳糖添加量为20g/L，接种量为5.0%，发酵温度为37 ℃，发酵时间为24 h。最后，利用薄板层析法测定多糖样品中含有的单糖，初步确定有葡萄糖、半乳糖、鼠李糖和甘露糖，多糖的抗氧化性研究结果表明, 保加利亚乳杆菌多糖样品液具有体外清除 O2 -·和·OH的作用, 在所作实验范围内其最大清除率分别为68.7%和 69.4%。 关键字：乳酸细菌 胞外多糖 薄板层析 发酵条件 氧化性 Abstract Lactic acid bacteria (LAB) extracellular polysaccharide (EPS) is secreted by the name of lactic acid bacteria from the cell wall of the generic capsular polysaccharide and mucus polysaccharides in the process of growth and metabolism. The experiments on extraction of exopolysaccharides from lactic acid bacteria in the fermentation broth by enzymatic method. By determination of extracellular polysaccharide were screened out 2 strains of polysaccharide high-yield extracellular, and the fermentation conditions optimization of single factor and orthogonal experiments, the optimum fermentation conditions optimization is: initial culture pH5.5, lactose concentration is 20g/L, inoculum 5%, fermentation temperature 37 ℃, fermentation time was 24 h. Finally, determination of monosaccharides containing polysaccharide samples of glucose, galactose, rhamnose and mannose by thin layer chromatography, antioxidant activity of polysaccharides showed that, Lactobacillus bulgaricus polysaccharide liquid samples with scavenging O2 - · and · OH role in the experimental range, the maximum clearance rates were 68.7% and 69.4%. Keywords: lactic acid bacteria Extracellular polysaccharide Thin layer chromatography Fermentation conditions Oxidation 目录 TOC \o 1-3 \h \u HYPERLINK \l _Toc7012 一、综述 PAGEREF _Toc7012 1 HYPERLIN