遗传重到组和转座子.ppt

* a、 RecA 有单、双链 DNA 结合活性,其中，双链结合活性最终导致的也是 RecA 与单链的结合状态 * RecA-mediated strand exchange between partially duplex and entirely duplex DNA generates a joint molecule with the same structure as a recombination intermediate. * The natural migration rate of a holiday junction is relatively slow. In E. coli the proteins Ruv A and Ruv B act as ‘motors’ to drive the migration faster. - ATP dependent process * Λphage进入E.coli后整合到寄主染色体上以及从染色体上切除均 attP 含 POP’三序列240bp（－160～＋80） Sne－－－235bp（－152～＋82） * ? 核心序列O全长15bp，富含A-T无碱基倒转对称性 ? Int结合 ----核心序列的反向位点（在每条链的适当位置上切割）