抗大豆花叶病毒 SMV 新基因挖掘及应用研究 The Exploration of.pdfVIP

6th OAPS Working Paper Series Paper No. 2009-034 Website: http://www.oaps.hk/ 抗大豆花叶病毒SMV 新基因挖掘及应用研究 The Exploration of New Resistant Gene and Applied Research For Soybean Mosaic Virus (SMV) 冯荔 顾士雄 Feng Li Gu Shixiong 农业与生物学院 School of Agriculture and Biology 摘要 多年生野生大豆与栽培大豆是近缘种，引入多年生野生大豆对我国大豆种植有深远的意义。本 研究针对我国大豆的主要病害大豆花叶病毒，大豆花叶病毒会导致大豆严重减产和种质衰退， 如今已成为世界范围内广泛分布并普遍发生的病毒病害之一。应用抗病品种是控制该病最经济有效 的办法。本研究首先将从国外收集到的Glycine 亚属的11 个种的78 份多年生野生大豆进行繁殖， 然后进行抗大豆花叶病毒的筛选。本文解决了在种子材料极少条件下，繁殖多年生野生大豆困难， 成苗率可达99% 以上，并成功筛选出珍贵的抗病材料2 份，这对于今后挖掘抗大豆花叶病毒的新基 因以及进一步的研究提供了依据。 关键词：多年生野生大豆，引种，繁殖，抗大豆花叶病毒，品种选育 ABSTRACT Wild (Glycine Soja) and cultivated (Glycine Max) soybeans are related, so implanting more useful genes of G. Soja has profound significance. Soybean Mosaic Virus (SMV) is a virus that harms the regular growth of soybean. Infection by SMV may result in severe yield losses and quality reduction. It has been one of the most prevalent plant viral pathogens in the world. The most effective and economical approach to avoiding the infection of SMV is to select out those species with genetic resistance to the virus. In this experiment, we cultivated 78 samples of G. Soja from 11 species in the Glycine subgenus and selected out those who bear the genes resisting the SMV. This paper is focused on the new efficient method that is designed to propagate G.soja from limited seeds and result in a seedling percentage above 99%. Selection was then made to obtain two valuable species that bear virus-resistant genes, which could help the further researches on exploring new virus-resistant genes. KEY WORDS: perennial Glycine soja, seed introduction, propagation,