桂花叶的化学成分及抗肿瘤活性研究-热带亚热带植物学报.PDFVIP

热带亚热带植物学报 2018, 26(2): 178 ~ 184 Journal of Tropical and Subtropical Botany 桂花叶的化学成分及抗肿瘤活性研究 1 2 1 1 3* 尹伟 , 郁阳 , 马秋丽 , 陈婷 , 张国升 (1. 安徽新华学院药学院，合肥 230088 ；2. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，昆明 650201；3. 安徽 中医药大学药学院，合肥 230031) 摘要：为了解桂花(Osmanthus fragrans)叶片的化学成分，应用多种色谱分离手段从桂花叶醇提溶液分离得到13 个化合物, 经 波谱分析分别鉴定为香橙素 (1) 、染料木黄酮 (2)、lupinalbin A (3) 、齐墩果酸甲酯 (4)、3-羰基齐墩果酸 (5) 、fouquierol (6)、 19α-羟基-3-O-乙酰熊果酸 (7) 、白桦脂醇 (8) 、豆甾-3,5-二烯-7-酮 (9) 、刺五加酮 (10) 、对羟基苯乙酮 (11) 、3,16α-二羟基- 对映贝壳杉烷 (12)和trigoflavidone A (13) 。所有化合物均为首次从桂花中分离得到。采用MTT 法检测抗肿瘤活性表明，化 合物2 、3 、10 均有一定的抗肿瘤活性，且对4 种肿瘤细胞株的活性检测为首次报道。 关键词：桂花; 叶片; 化学成分; 结构鉴定; 抗肿瘤活性 doi: 10.11926/jtsb.3786 Study on Chemical Constituents and Antitumor Activities of Leaves of Osmanthus fragrans 1 2 1 1 3* YIN Wei , YU Yang , MA Qiu-li , CHEN Ting , ZHANG Guo-sheng (1. Department of Pharmacology, Xinhua College of Anhui, Hefei 230088, China; 2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany , Chinese Academy of Sciences, Kunming 650201, China; 3. Department of Pharmacology, Anhui University of Traditional Chinese Medicine, Hefei 230031, China) Abstract: In order to understand the chemical constituents of Osmanthus fragrans leaves, thirteen compounds were isolated from the 95% ethanol extract. On the basis of spectral data, their structures were identified as aromadendrin (1), genistein (2), lupinalbin A (3), methy oleanolate (4), 3-ketooleanolic acid (5), fouquierol (6), 19α-hydroxyl acetylursolic acid (7), betulin (8), stigmasta-3,5-dien-7-one (9), ciwujiatone (10), p -hydroxyaceto- phenone (11), entkaurane-3,16α-diol (12), and trigoflavidone A (13). All of compounds are isola