基于亲和层析两种蛋白质纯化策略.pptVIP

3 Tandem Affinity Tag 钙离子螯合剂 钙调素结合多肽 烟草蚀纹病毒蛋白酶 4 Conclusions and Inspiration 1. Self-cleaving tags have the potential to have a significant impact on protein purification in the commodity enzyme and human therapeutic industries, and can be generalized for use with virtually any target protein. 2. TAP approache is therefore directed to the production of a highly pure protein for e.g., crystallization but are also widely used for the isolation of protein complexes to study protein interactions. 3. Future innovation…… 4. Inspiration: choose the approach by your needs 1. A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions. 2. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. 3. An efficient protein complex purification method for functional proteomics in higher eukaryotes. 4. The potential role of self-cleaving purification tags in commercial-scales process. 5. Intein Applications From Protein Purification and Labeling to Metabolic Control Methods. 6. A set of ligation-independent in vitro translation vectors for eukaryotic protein production. 7. A generic protein purification method for protein complex characterization and proteome exploration References: . Intranuclear functions · involve in general transcriptional or epigenetic machinery;(和RNA聚合酶,甲基转移酶，乙酰转移酶等共同作用。) · involve in general inflammatory functions;（骨髓细胞中，IL-6和IL-10都能激活STAT3，分别引起促炎症反应和抗炎症反应 ） · involve in lineage commitment（在T细胞中广泛研究。具体机制尚待深挖） ·Extranuclear functions: · promote oxidative phosphorylation and membrane permeability（STAT3，线粒体中。除了STAT4，其他家族成员也都在线粒体中被监测到过，但功能尚不明。） * Con1：a linker region including a specific sequence for endoprotease cleavage increase accessibility of the affinity tag and is often required for effective endoprotease cleavage Con2：aid solubility and folding like maltose-binding protein (MBP) , glutathione S-transferase (GST) or small ubiquitin modifying protein (SUMO), thioredoxi