基于iTRAQ技术的艾滋病热毒蕴结证与脾肾阳虚证 的蛋白组学研究-中医内科学专业论文.docxVIP

摘要 目的 探索艾滋病期热毒蕴结证、脾肾阳虚证患者与健康人之间蛋白质差异表达， 为艾滋病上述中医证型临床诊断的客观化提供依据。 方法 运用相对和绝对定量同位素标记(isobaric tags for relative andabsolute quantitation， iTRAQ) 技术分别对采集的艾滋病期热毒蕴结证、脾肾阳虚证患者和健康对照组 血液标本进行差异蛋白检测。采用 SPSS17.0 统计软件分析年龄、性别等计数或 计量资料，用 Thermofisher Proteome Discoverer 1.3 对各组差异蛋白进行筛选。 结果 .热毒蕴结证与对照组之间共筛选出差异蛋白 184 个（差异倍数大于 1.2）， 其中上调的有 94 个，下调的有 90 个。其涉及的功能主要有防御反应的调控、急 性炎症反应的调控、免疫效应过程的正态调控、白细胞免疫介导的调节、免疫球 蛋白介导免疫反应、外部刺激的应答、B 细胞介导免疫等。 .脾肾阳虚证与对照组之间共筛选出差异蛋白 174 个（差异倍数大于 1.2）， 其中上调的有 79 个，下调的有 95 个。其涉及的功能主要有氧化还原酶的活动、 过氧化物酶活性、调节内皮细胞增殖、信号传导的调控、抗氧化活性、调节细胞 内的蛋白激酶级联、催化活性等。 结论 1.艾滋病期热毒蕴结证患者和健康对照组存在差异蛋白表达，complement C3 precursor （上调）为辅助该证型临床诊断的潜在蛋白。 2.艾滋病期脾肾阳虚证患者和健康对照组存在差异蛋白表达， hemoglobin delta（下调）为辅助该证型临床诊断的潜在蛋白。 1 关键词 艾滋病；热毒蕴结证；脾肾阳虚证；iTRAQ；蛋白 2 Abstract Objective Find AIDS accumulation of Heat-toxin Stagnation Syndrome and Spleen-Kidney Yang Deficiency Syndrome protein differences between patients and healthy people to express, and for the clinical diagnosis of AIDS the TCM syndrome type of objective provides the basis. Methods The blood samples of Heat-toxin Stagnation Syndrome Spleen-Kidney Yang Deficiency Syndrome and health control group are detected to find difference Protein expressions based on iTRAQ technology.Age, gender and other count or measurement data are Analysised by SPSS17.0 statistical software.Difference expression proteins of each group are choosed by Thermofisher Proteome Discoverer 1.3. Results Between the Heat-toxin Stagnation Syndrome and the control group were screened out proteins 184（fold difference greater than 1.2), which raised the 94, the cut is 90. The functions involved are mainly normal regulation, white blood cell immune mediated regulation of defense responses, acute inflammatory response of the immune effect and the regulation of process regulation, immune globulin protein mediated by immune response, external stimuli response, cell - mediated B guide immune. Spleen-Kidney Yang Deficiency Syndrome and the control group were screened out proteins 174 （ fo