应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗病菌.PDF

中国水稻科学(Chin J Rice Sci), 2018, 32(5): 493 －500 DOI: 10.16819/j.1001-7216.2018.7119 493  应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗 病菌 1 1 1 2 3 1,* 袁咏天 戎振洋 叶文武 张红生 庄义庆 郑小波 1 2 3 * ( 南京农业大学 植物保护学院，南京 210095 ；南京农业大学 农学院，南京 210095 ；镇江市农业科学院，江苏 镇江 212400 ；通讯联系人，E-mail: xbzheng@) Detection of Seed-borne Rice Bakanae Pathogens in Jiangsu Province, China Using Loop-mediated Isothermal Amplification Assays 1 1 1 2 2 1,* YUAN Yongtian , RONG Zhenyang , YE Wenwu , ZHANG Hongsheng , ZHUANG Yiqing , ZHENG Xiaobo (1 College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 , China; 2 College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China; 3Zhenjiang Agric-academy of Sciences, Zhenjiang 212400, China; Corresponding author, Email : xbzheng@) Abstract: 【Objective 】The research aims to reveal the situation of seed-borne rice bakanae pathogens in Jiangsu Province, China. 【Methods 】The seeds of 65 major rice cultivars collected from this area were analyzed by using loop-mediated isothermal amplification (LAMP) assays for specific detection of 4 known rice bakanae pathogen species, including Fusarium fujikuroi, F. proliferatum, F. verticilliodes and F. andiyazi . 【Results 】There were 54 rice cultivars carrying rice bakanae pathogens, indicating it’s a common phenomenon that rice seeds carrying rice bakanae pathogens in Jiangsu Province. And the most frequent species was F. fujikuroi, representing 83.07% of the total samples tested, followed