茶多酚葡萄柚提取物联合应用对变异链球菌生长产酸的影响黄晓晶.DOC

[作者简介：黄晓晶（1971-），女,福州人,博士,主任医师,硕士研究生导师,主要从事龋病学研究。E-mail: hxiaoj@163.com,电话：（0591基金项目]：福建省医学创新课题（2009-CXB-36）。 茶多酚葡萄柚提取物联合应用对变异链球菌生长、产酸的影响 黄晓晶，卢国英，刘建党，江 山，雷丽珊（350002福州，福建医科大学附属口腔医院什么科室？） [摘要] 目的 研究茶多酚与葡萄柚提取物联合应用对变异链球菌生长、产酸的影响。方法 采用棋盘稀释法测定茶多酚葡萄柚提取物混合液对变异链球菌的最低抑菌浓度（MIC）值，计算分级抑制浓度(FIC)指数来判定二者的联合效应；并测定低于MIC浓度的茶多酚葡萄柚提取物混合液对变异链球菌产酸的影响。结果 茶多酚与葡萄柚提取物联合应用时的MIC值较二者单独应用时下降，FIC=0.75结果太简单？。结论 茶多酚与葡萄柚提取物联合应用对变异链球菌的体外抗菌活性具有相加作用，较各自的单组份用药在更低浓度即可有效抑制变异链球菌的生长、产酸。 结果太简单？ [关键词] 茶多酚；葡萄柚提取物；变异链球菌；抗菌活性；棋盘法 [中图法分类号] R781.1 [文献标识码] A Effect of combined application of tea polyphenols with grapefruit-seed extract on growth and acid production of Streptococcus mutans Huang Xiaojing, Lu Guoying, Liu Jiandang, Jiang Shan, Lei Lishan (School of Stomatology, Fujian Medical University, Fuzhou 350002, Abstract：Objective To investigate the effect of combined application of Tea Polyphenols (TP) and Grapefruit-seed extract (GSE) on growth and acid generation of Streptococcus mutans. Methods Minimal inhibitory concentration (MIC) of combined application of TP with GSE on growth of Streptococcus mutans ATCC 25175 was measured with Checkerboard method. Fractional inhibitory concentration (FIC) was calculated accordingly to evaluate the combined effect of TP and GSE. The effect on acid production of Streptococcus mutans ATCC 25175 of combined application of TP and GSE with concentration lower than MIC was also observed. Results Compared with application of TP or GSE alone, The combined application of TP and GSE possess lower MIC with FIC at 0.75. Conclusion Combined application of TP and GSE has additive antibacterial effect on Streptococcus mutans. Compared with application of TP or GSE alone, combined application of TP and GSE could inhibit the growth and acid production of Streptococcus mutans at lower concentration. Key Words：Tea Polyphenols; Grapefruit-seed Extract; Streptococcus mutans; Antimicrobial activity; Checkerboard method 含漱剂控制菌斑已成为患者自我菌斑控制从而达到防龋效果的重要方法之一。以往临床普遍使用的治疗