罗格列酮抑制粘附分子表达减轻大鼠急性心肌梗死再灌注后无再流.doc

编号： 　　 刊用形式：论著 文章编号：200804019 罗格列酮抑制粘附分子表达减轻大鼠急性心肌梗死再灌注后无再流 何 泉，雷 寒，覃 数，马康华 （重庆医科大学，附属第一医院心内科，重庆 400016 ） 摘要： 目的 观察罗格列酮对大鼠急性心肌梗死（acute myocardial infarction，AMI）再灌注后无再流的影响及其机制。方法 45只SD大鼠随机分为假手术组、生理盐水组、罗格列酮组（3 mg·kg-1·d-1, 共14 d），每组15只，后2组大鼠行冠状动脉结扎3 h，松解2 h建立AMI再灌注后无再流模型。以病理染色法检测无再流面积；化学比色法检测再灌注区心肌髓过氧化物酶（myeleperoxidase， MPO）活性；以免疫组化及RT-PCR方法检测再灌注区细胞间粘附分子-1（Intercellular adhesion molecule-1，ICAM-1）和P-选择素蛋白及mRNA的表达。结果①（1）罗格列酮组心肌无再流范围较生理盐水组显著减小（P 0.01）；②（2）生理盐水组心肌MPO酶活性、ICAM-1及P-选择素蛋白及mRNA表达水平较假手术组均显著升高（P 0.01）,罗格列酮组上述指标较安慰剂组显著降低（P 0.05）。结论 罗格列酮能有效的减小大鼠急性心肌梗死再灌注后无再流范围，其机制可能与抑制再灌注后内皮ICAM-1及P-选择素的表达，进而减少中性粒细胞的粘附及聚积有关。 关键词：无再流现象；心肌梗死；罗格列酮；细胞间粘附分子-1；P-选择素 中图分类号：R541.4 文献标识码：A Rosiglitazone reduced the area of no-reflow after AMI with reperfusion in rats by inhibiting the expression of adhesion molecules HE Quan, LEI Han, QIN Shu, MA Kang-Hua.(Department of Cardiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016 ) Abstract：Objective To investigate the effect and mechanism of rosiglitazone on no-reflow after acute myocardial infarction with reperfusion in rats. Methods 45 SD rats were randomly divided into sham-operated group、normal saline treated group and rosiglitazone treated group(3 mg·kg-1·d-1, for 14 days), 15 rats for each group; To establish no-reflow model, the rats of the latter two groups received coronary artery ligation for 3 h with reopening for 2 h; And pathological staining for no-reflow、chemical assay for MPO activity、immunohistochemistry and RT-PCR for proteins and mRNA expression of ICAM-1 and P-selectin in myocardium were performed. Results (1) Area of no-reflow in rosiglitazone treated group was significantly reduced ,compared with that in normal saline treated group (P 0.01)；(2) the MPO activity、proteins and mRNA expression of ICAM-1 and P-selectin in normal saline treated group were significantly increased (P0.01), compared with that in