活性维生素D对2型糖尿病大鼠肾组织胰岛素信号通路PI3KAkt的影响.docVIP

活性维生素D对2型糖尿病大鼠肾组织胰岛素信号通路PI3K/AKT的影响 邓娟娟 张丹 韩晴 孔亚婷 朱慧铭 朱正庭 叶明 彭艳 潘希峰 李书国 （三峡大学第一临床医学院，湖北宜昌市中心人民医院老年病科，湖北宜昌443003） 【摘要】 目的 探讨活性维生素D对2型糖尿病大鼠肾组织胰岛素相关信号分子胰岛素受体（IRβ）、磷脂酰肌醇3激酶( PI3K)、蛋白激酶B(Akt／PKB)表达的影响。 方法 30只雄性Wistar大鼠随机分为普通饲料组即正常组（N组 10只）和高脂高糖饲料组（20只），高脂高糖饲料组以小剂量(30mg/kg)链脲佐菌素（STZ）加高糖高脂饮食诱导2型糖尿病模型后随机分为2组，分别经腹腔注射2.5ug/kg.d-1花生油（M 组,10只）或腹腔注射2.5ug/kg.d-1溶于花生油中的活性维生素D（D 组，10只)。8周后留取大鼠血标本，测定血糖、血肌酐及尿素氮,处死大鼠留取肾组织。Western Blot 检测肾组织中胰岛素相关信号分子IRβ、 PI3K、Akt的表达及其磷酸化的水平。结果 糖尿病组大鼠血糖、尿素氮、肌酐高于正常（P0.05)）；而相对于糖尿病组，维生素D干预组血糖、尿素氮及肌酐明显降低（P0.05）. Western Blot显示相较于正常组，糖尿病组大鼠肾组织中三组总的IRβ、Akt的表达无明显改变而其磷酸化水平及PI3K的水平明显下调，而维生素D干预后明显上调(P0.05).结论　活性维生素D可激活胰岛素信号通路PI3K/AKT的表达，对糖尿病大鼠肾脏损伤发挥保护作用。 【关键词】1, 25-二羟胆骨化醇；糖尿病；PI3K/AKT The impact of 1, 25-dihydroxyvitamin D3 on insulin signal path about PI3K/AKT on kidney tissue of type 2 diabetic rats DENG Juan-juan; ZHANG Dan; HAN Qin; KONG Ya-ting ;ZHU Hui-ming;ZHU Zheng-ting; YE Ming;PENG Yan;PAN Xi-feng；LI Shu-guo Department of Geriatrics,First College of Clinical Medical Science,China Three Gorges University,Central Peoples Hospital of Yichang,Yichang443003, Hubei, China 【Abstract】 Objective To study the impact of 1, 25-dihydroxyvitamin D3 on insulin signal path about PI3K/AKT on kidney tissue of type 2 diabetic rats. Methods 30 wistar rats were divided into normal(10 rats ) and experimental groups randomly(20 rats). Wistar rats of experimental group were injected low dose streptozotocin (25mg/kg) and were fed with diets enriched in both glucose and fat to form type 2 diabetic model,then were divided into 2 groups randomly.And each group had 10 diabetic rats. 1, 25-dihydroxyvitamin D3(Vitamin D group) or peanut oil in the same dose as 1, 25-dihydroxyvitamin D3 ( Diabetic group) was injected into diabetic rats through enterocoelia, then killed all rats after 8 weeks. The levels of blood sugar(BS) ,biood urea nitrogen(BUN) and serum creatinine(Scr) were measured. The expression of P-IR,P-AKT and PI3K were detected by Western blot. Resul