脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其影响.docVIP

脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其影响 邹 佳，李长清 （重庆医科大学附属第二医院神经内科，重庆400010） 摘要：目的 观察侧脑室注射NEP1-40对脑缺血再灌注大鼠轴突再生、患肢功能恢复和RhoA信号通路活动的影响，探讨其可能的机制。方法 将60只成年雄性Sprague-Dawley大鼠分为假手术组、脑缺血再灌注组、侧脑室注射PBS组和侧脑室注射NEP1-40组；采用线栓法制作大鼠大脑中动脉缺血再灌注模型；用Western blot方法检测缺血灶周边脑组织生长相关蛋白（GAP-43）和RhoA蛋白表达；用Montoya设计的“楼梯测试”方法对患肢运动功能进行测试。结果 （1）侧脑室注射NEP1-40组大鼠缺血灶周边脑组织GAP-43在术后7d和14d均高于脑缺血再灌注组和侧脑室注射PBS组，以术后7d最显著。（2）侧脑室注射NEP1-40组大鼠缺血灶周边脑组织RhoA在术后各时相点显著低于脑缺血再灌注组和侧脑室注射PBS组。（3）侧脑室注射NEP1-40组大鼠在术后各时相点“楼梯测试”结果显著高于脑缺血再灌注组和侧脑室注射PBS组。结论 NEP1-40能够促进脑缺血再灌注大鼠损伤后轴突再生和运动功能恢复，其机制可能与抑制RhoA信号通路活动有关。 关键词:脑缺血再灌注；NEP1-40；生长相关蛋白-43；RhoA 中图法分类号： 文献标识码:A The effect of NEP1-40 on GAP-43 and RhoA expression in rats of cerebral ischemic-reperfusion model ZOU Jia, LI Chang-qing(Department of Neurology, the Second Affiliated Hospital of Chong qing University of Medical Sciences, Chong qing 400010, China) Abstract ：Objective To study the effects of NEP1-40 on axon regeneration, motor function recovery of affected limb and RhoA signal pathway in cerebral ischemia-reperfusion rats, for exploring its possible mechanism. Methods Totally 60 adult male Sprague-Dawley(SD) rats were equally divided into sham operation group(sham group),cerebral ischemia-reperfusion control group(control group),intra-lateral ventricle injection of PBS group(PBS group) ,intra-lateral ventricle injection of NEP1-40 group(NEP1-40 group). The middle cerebral artery ischemia-reperfusion model (MCAI/R) was established by nylon monofilament occlusion method in rats. The changes of growth associated protein(GAP-43) and RhoA expression were determined by Western blot technique. The motor function of affected limb was tested by the “staircase test” of Montoya’s design. Results （1）The expression of GAP-43 in NEP1-40 group were higher than that in control and PBS group at 7d and 14d after MCAI/R，especially at 7d.（2）The expression of RhoA in NEP1-40 group were much lower than that in control and PBS group at each