分离纯化柿子多糖的树脂筛选及纯化工艺的响应面优化-中国农学通报.PDF

中国农学通报 2012,28(09):270-275 Chinese Agricultural Science Bulletin 分离纯化柿子多糖的树脂筛选及纯化工艺的响应面优化 李宇华，金文斌，郭琳博，陈湘宁 （北京农学院食品科学与工程学院，北京102206） 摘 要：为优化大孔树脂纯化柿子多糖工艺，在对8 种大孔树脂进行筛选的基础上，采用三因素三水平响 应面法，以动态吸附率和解吸附率为指标进行试验，最终所得优化结果为：树脂AB-8 具有较好的分离纯 化柿子多糖的性能。优化后的动态吸附试验条件为，样液浓度4.34 mg/mL ，上样速率1.89 BV/h ，样液体 积3.41 BV （柱体积）；解吸附试验条件为：洗脱剂中乙醇浓度55.41% ，洗脱速率3.50 BV/h ，洗脱剂用量 3.54 BV 。此条件下，动态吸附率和解吸附率的预测值分别达到89.87%和96.59% ，验证值误差均在5% 左右。 关键词：大孔树脂；柿子多糖；吸附；解吸附；响应面分析 中图分类号：TS202.3 文献标志码：A 论文编号：2012-0006 Screening Macroporous Resin for Separation and Purification of Polysaccharide from Persimmon and Optimization of Purification Technology by Response Surface Methodology Li Yuhua, Jin Wenbin, Guo Linbo, Chen Xiangning (Faculty of Food Science, Beijing University of Agriculture, Beijing 102206) Abstract: To optimize the technology for purifying polysaccharide from persimmon with macroporous resin, 8 kinds of macroporous resins were screened and response surface methodology (RSM) based on three levels and three factors was adopted to optimize the optimal levels by taking the dynamic adsorption and desorption rate as indices. The results showed: resin AB-8 had the best separation purification capability for persimmon polysaccharides; the sample liquid concentration 4.34 mg/mL, sample rate 1.89 BV/h, sample volume 3.41 BV (column volume); desorption test conditions: ethanol concentration 55.41% of eluting agent, the elution rate 3.50 BV/h, elution dosage 3.54 BV. Under this condition, the predicted dynamic adsorption and desorption rate respectively were 89.87% and 96.59%, the testing experiment result showed both relative errors were about 5%. Key words: macroporous