超声致微泡声孔效应增强兔微泡声孔效应增强兔-南方医科大学学报.PDF

J South Med Univ, 2013, 33(9): 1377-1381 doi 10.3969/j.issn. 1673-4254.2013.09.26 ·1377 · 基础研究 超声致超声致微泡声孔效应增强兔微泡声孔效应增强兔VXVX 肿瘤细胞通透性的适宜声学肿瘤细胞通透性的适宜声学 22 参参数数 罗利红，吴凤林，王宝平，许婷婷，胡 洁，隋 洋 南方医科大学南方医院超声诊断科，广东 广州 510515 摘要：目的探讨超声治疗仪不同声强、脉冲宽度、辐照时间和不同微泡剂量下声孔效应增大肿瘤细胞膜通透性的适宜声学参 数。方法 将90 个接种于新西兰白兔的VX2 移植瘤按声强、脉冲宽度、辐照时间和微泡剂量分为4 个部分，各部分设有不同水 平。治疗超声直接辐照肿瘤体表处，同时经静脉通道连续、缓慢注入微泡。治疗结束后立即取材制作组织切片，利用硝酸镧示 踪电镜观察肿瘤细胞通透性变化，依次得出4 个因素的适宜声学参数。结果 超声联合微泡辐照致声孔效应能不同程度增大毛 细血管壁通透性，其适宜的声强、脉冲宽度、辐照时间及微泡剂量分别为0.43 W/cm2 、2.42 ms、10 min、0.50 ml/kg。结论 超声联 合微泡辐照致声孔效应能透过毛细血管壁将肿瘤细胞膜“打孔”，有望增强肿瘤化疗效果。 关键词：声孔效应；超声；微泡；通透性 Optimization of acoustic parameters of microbubble-enhanced ultrasound sonoporation for augmenting tumor cell permeability in vivo LUO Lihong, WU Fenglin, WANG Baoping, XU Tingting, HU Jie, SUI Yang Department of Ultrasound, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To explore the appropriate acoustic parameters for augmenting the permeability of tumor cells using microbubble-enhanced ultrasound. Methods Ninety intramuscular VX2 tumors grown in New Zealand white rabbits were randomly divided into 4 parts by 4 factors, namely sound intensity, pulse width, insonation time and microbubble dose, each at different levels. Therapeutic ultrasound was delivered directly to the tumor surface during intravenous infusion of microbubbles. The tumor was excised after the treatment and sliced to investigate the change in tumor cell permeability using lanthanum nitrate electron microscopy. The appropriate acoustic parameters of the 4 factors were investigated. Results Sonoporation induced by microbubble-enhanced ultrasound augmented the permeability of the tumor cells, and the appro- priate sound intensity, pulse width, insonation time and microbubble dose were 0.