肿瘤坏死因子-α单核苷酸多态性与口腔扁平苔藓的相关性研究.docVIP

肿瘤坏死因子-α单核苷酸多态性与口腔扁平苔藓的相关性研究 柏景坪1，王红健1，付 洁1，陈方淳2 (100050北京，首都医科大学附属北京口腔医院牙周黏膜科1；400015重庆，重庆医科大学附属口腔医院牙周黏膜科，重庆市口腔疾病与生物医学研究中心2） [通信作者] 陈方淳，电话：（023E-mail：fangchun_chen@ [摘要] 目的 研究肿瘤坏死因子-α（TNF-α）与口腔扁平苔藓（oral lichen planus, OLP）的相关性。方法 应用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)检测151例OLP患者（包括81例糜烂型扁平苔藓和70例非糜烂型扁平苔藓）和143例健康对照者的TNF-α-308位点单核苷酸多态性，分析其基因型、等位基因和单元型频率，并进行关联分析。结果结果太简略，请包括正文主要结果。 糜烂型OLP组TNF-α-308位点的基因型和等位基因频率分布与对照组相比均有显著性差异（P0.05），且基因型频率的相对风险分析发现，TNF-α-308 TNF2基因型患糜烂型OLP的风险是TNF1的2.268倍(OR=2.268, P0.05)。结论 TNF-α基因启动子区-308位点的多态性可能不是影响OLP的一个独立因素，但TNF-α-308 *A等位基因可能是扁平苔藓局部炎症反应加重的危险因子之一。 结果太简略，请包括正文主要结果。 [关键词] 口腔扁平苔藓；基因多态性；单核苷酸多态性；TNF-α [中图法分类号] [文献标志码] A Polymorphisms of TNF-α gene and the susceptibility of oral lichen planus Bai Jingping1, Wang Hongjian1, Fu Jie1, Chen Fangchun2(1Department of Peridontics and Oral Medicine, Beijing Stomatology Hospital Affiliated to Capital University of Medical Science，Beijing, 100050;2Department of Peridontics and Oral Medicine, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science，Chongqing, 400015, China) [Abstract] Objective To explore the possible relationship between the single nucleotide polymorphisms of TNF-α and the susceptibility of oral lichen planus. Methods The polymorphisms of TNF-α-308 were detected in 151 OLP cases (including 81 erosive OLP and 70 nonerosive OLP)and 143 healthy controls by using the PCR-RFLP technique. Results Analyze between the erosive OLP group and the healthy group showed that the allele frequencies of TNF-α-308 *A was much higher in the erosive OLP group(P＝0.0130.05), and subjects carrying the TNF1 genotype had significantly increased risk of OLP(OR=2.268, P0.05). 对照中文修改Conclusion The polymorphisms of TNF-α-308 may be not the single dependent factor associated with the susceptibility and development of OLP, but the TNF-α-308 *A allele could attribute to evaluate the severity of OLP. 对照中文修改