两个棉花β-葡聚糖酶新基因的结构与表达特征分析-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2011, 37(1): 4047 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.00040 两个棉花-葡聚糖酶新基因的结构与表达特征分析 董 佳 蔡彩平 王立科 赵 亮 张天真 郭旺珍* / , 210095 : β- β-cDNA 2 β-, -1,4-β-(GhEG, GenBank HM462003) 1,3-β- (GhGLU, GenBank HM462004) GhEG ORF 1 581 bp, 526 GhGLU ORF 1 410 bp, 469 , GhEG 5 6 , GhGLU , 1 2 1 , 2 SNP GhEG GhGLU 1 19 4 Q-PCR , GhEG , , 15 DPA 20 DPA , 7124 TM-1 GhGLU , , , TM-1 7124 : -1,4-β-; 1,3-β-; ; Structure and Expression Analysis of Two Novel Genes Encoding β-glucanase in Cotton * DONG Jia, CAI Cai-Ping, WANG Li-Ke, ZHAO Liang, ZHANG Tian-Zhen, and GUO Wang-Zhen National Key Laboratory of Crop Genetics Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China Abstract: The β-glucanase is a type of enzyme degrading β-glucan. Cloning and expression analysis of genes encoding β-glucanase can provide information in both gene resources and breeding utilization for improvement of cotton fiber quality. The two novel genes encoding β-glucanase, designated as GhEG (GenBank accession No. HM462003) and GhGLU (GenBank acces- sion No. HM462004), were obtained by sillico cloning and cDNA library screening, respectively. GhEG contained an open read- ing frame of 1 581 bp that encoded a polypeptide of 526 amino acids, and GhGLU contained an open reading frame of 1 410 bp that encoded a polypeptide of 469 amino acids. The genome sequenc