药根碱对脂肪细胞糖脂代谢的影响及机制研究.docVIP

药根碱对脂肪细胞糖脂代谢的影响及机制研究 王慧，陈刘，姜友昭，陈兵 （第三军医大学西南医院内分泌科, 重庆400038） [摘要] 目的：探讨药根碱对3T3-L1脂肪细胞葡萄糖摄取、脂肪酸氧化的影响及可能作用机制。方法：药根碱作用于3T3-L1脂肪细胞48小时后，用[3H]2-DG标记检测胰岛素介导的葡萄糖摄取情况、14C-palmitic标记检测脂肪酸氧化情况；Real-time PCR检测PPARs的基因表达情况，Western blot检测PPARs的蛋白表达情况，采用PBS溶液作为对照组，其余实验条件相同。结果：药物毒性探索药根碱最佳作用浓度为0.15 mg/L，最佳作用时间为48小时；与PBS对照组相比，药根碱具有促进3T3-L1细胞脂肪酸氧化、PPARalpha、PPARbeta基因和蛋白表达的作用(P＜0.05或P＜0.01),而对PPARgamma基因和蛋白表达无明显影响。结论：药根碱对3T3-L1细胞脂肪酸氧化具有显著促进作用，其可能机制为上调PPARalpha和PPARbate基因和蛋白表达 [关键词]药根碱、3T3-L1、葡萄糖摄取、脂肪酸氧化、PPARs、PPARalpha、PPARbate、PPARgamma。 Effect and mechanism of Jatrorrhizin on glucose uptake and fatty acid oxidation in adipocytes WANG Hui, CHEN Liu, JIANG You-zhao，CHEN Bing (Department of Endocrinology, Southwest Hospital，Third Military Medical University, Chongqing 400038) Abstract: Objective To detect glucose uptake,fatty acid oxidation,mRNA and protein expression of the nuclear receptors peroxisome proliferator-activated 基金项目：十一五国家科技支撑计划地方专项（项目批准号：2007BA14B03） 作者简介：王慧（1983-），女，山东人，硕士研究生，主要从事内分泌与代谢疾病方面的研究。 电话E-vmail： HYPERLINK mailto:whyitong@126.com whyitong@126.com 通讯作者：陈兵，电话（023E-mail： HYPERLINK mailto:chenbing3@ chenbing3@ receptors in 3T3-L1 adipocytes coculture with Jatrorrhizin,then discuss the effect and mechanism of Jatrorrhizin on glucose uptake and fatty acid oxidation of adipocytes. Methods：3T3-L1 adipocytes coculture with Jatrorrhizin for 48 hours,insulin stimulates glucose uptake in 3T3-L1 adipocytes was detected by 3H-labeled deoxyglucose,fatty acid oxidation in 3T3-L1 adipocytes coculture was detected by 14C-labected palmitic, the genes expression of PPARs were determined by real-time PCR,and the proteins expression were determined by western blot. Results：The best effect concentrotion of Jatrorrhizin is 0.15 mg/L,the best reaction time is 48 hour Fatty acid oxidation in 3T3-L1 adipocytes in vitro was promoted by Jatrorrhizin,and the gene and protein expression of PPARalpha and PPARbate were up-regulation, no-regulation of PPARgamma never