溶组织内阿米巴.pptVIP

Entamoeba histolytica 溶组织内阿米巴 Trophozoite Trophozoite (Haematoxylin Eosin stained) Uninucleated Cyst (iron-haematoxylin stained) 2-nucleated Cyst (iron-haematoxylin stained) 4-nucleated Cyst (iron-haematoxylin stained) Cyst (Iodine stained) Host : man Infective stage: mature cyst Basic process in life history: cyst - trophozoite - cyst Parasitic location: colon Route of infection: per-oral Pathogenic stage: trophozoite 1. Intestinal amoebiasis Liver abscess Source of infection : patients with chronic dysentery and carriers. Way and route to get infection : to ingest food or water contaminated by 4-nucleated cyst. 1.Fresh human feces should not used as fertilizer. 2.Drinking water must be boiled . 3.Food and drinks must be protected from flies. 4.Pay attention to personal hygiene. Questions 1. Explain the terms: trophozoite ; cyst; protozoa 2. The classification of protozoa 3. The life cycle of Entamoeba histolytica. 4. The summery of the life cycle. 5. How to diagnose intestinal amoebiasis? Epidemiology Prevention * * E.histolytica is the only pathogenic amoeba among all of the intestinal amoeba, causing amoebic dysentery 阿米巴肠炎 or extraintestinal amoebiasis 肠外阿米巴病 (cerebral amoebiasis, amoebic liver abscess, lung abscess). Perhaps 10% the worlds population are infected. Preface E.histolytica inhabits colon结肠. Human are the most suitable hosts, but apes, dogs, mice, pigs have been proved to be parasitized. In its life cycle, there are 2 stages ( trophozoite and cyst). Preface  Trophozoite 滋养体 Cyst 包囊 Morphology Ectoplasm Erythrocyte in food vacuole Nucleus Nucleolus 核仁 Endoplasm 12-60μm RBC Nucleus Nucleolus Trophozoite (Haematoxylin Eosin stained) Trophozoite Cyst Chromatoid body 拟染色体 Glycogen vacuoles 糖原泡 Immature Cyst Mature Cyst Chromatoid body nuclei Chromatoid body Glycogen vacuoles 4-nucleated trophozoite mature